{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:02:47Z","timestamp":1785402167321,"version":"3.56.0"},"posted":{"date-parts":[[2025,12,1]]},"group-title":"Cell Biology","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01 DK134343"],"award-info":[{"award-number":["R01 DK134343"]}],"id":[{"id":"https:\/\/ror.org\/00k86s890","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01DK114126"],"award-info":[{"award-number":["R01DK114126"]}],"id":[{"id":"https:\/\/ror.org\/00k86s890","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Crhn\u2019s & Colitis Foundation Senior Research Award","award":["902766"],"award-info":[{"award-number":["902766"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background and aims<\/jats:title>\n                  <jats:p>Transfer RNA (tRNA) modifications determine translation fidelity and efficiency. It occurs through the action of specific enzymes that modify the nucleotides within the tRNA molecule. Our previous study demonstrated tRNA modopathies and altered queuine-related metabolites in inflammatory bowel diseases. Queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1) and QTRT 2 co-localize in mitochondria and form a heterodimeric TGT participating in tRNA Queuosine (tRNA-Q) modification. Human body acquires Queuine\/Vitamin Q from intestinal microbiota or from diet. However, the roles of tRNA-Q modifications in the maintenance of intestinal mitochondrial homeostasis and microbiome are still unclear.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    We used publicly available human IBD datasets, QTRT1 knockout (KO) mice, QTRT1 intestinal epithelial conditional KO (QTRT1\n                    <jats:sup>\u0394IEC<\/jats:sup>\n                    ) mice, cultured cell lines with QTRT1-specific siRNA, and organoids from patients with IBD to investigate the mechanism of tRNA-Q modifications in intestinal mitochondrial homeostasis and therapeutic potential in anti-inflammation.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    In single cell RNA sequencing datasets of human IBD, we identified significant reduced intestinal epithelial QTRT1 expression in the patients with Crohn\u2019s Disease. Using publicly available datasets, we identified significantly changes of Vitamin Q-associated bacteria in human IBD, compared to the healthy control. Qtrt1\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice had significant reduction of Q-associated bacteria, e.g.,\n                    <jats:italic>Bacteroides<\/jats:italic>\n                    . Alcian Blue and Mucin-2 staining revealed mucosal barrier damage and disrupted homeostasis, with reduced colonic cell proliferation. Intestinal tight junction integrity was impaired in QTRT1-KO mice, as evidenced by reduced ZO-1 and increased Claudin-10 expression. QTRT1\n                    <jats:sup>\u0394IEC<\/jats:sup>\n                    mice also showed dysbiosis and disrupted TJs. ATP synthesis was significantly decreased in the colon of QTRT1-KO mice, accompanied by severe mitochondrial dysfunction: reduced mitochondrial quality, Cytochrome-C release, and mitochondrial DNA (mtDNA) leakage. Mitochondrial dysfunction contributed to colonic cell death, as shown by elevated expressions of Cleaved Caspase-3 and Cleaved Caspase-1, increased BAX\/Bcl-2 ratio, and positive TUNEL signals. Elevated CDC42, CD14, and CD4 levels in QTRT1-KO colon suggested mucosal immune activation and tissue repair responses. QTRT1-deficient CaCO2-BBE cells showed mitochondrial dysfunction. Cytochrome-C and mito-DNA release leading to cell death characterized by elevated expressions of Cleaved Caspase-3 and Caspase-1, increased BAX\/Bcl-2 ratio, and higher apoptosis rate. Organoids isolated from patients with IBD showed reduced levels of QTRT1 and dysfunctional mitochondria. Restoring mitochondrial function leads to enhanced QTRT1.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>These findings underscore the critical role of QTRT1\/Q-tRNA modification in maintaining intestinal and microbial homeostasis. Mechanistically, QTRT1 loss impacts mitochondrial integrity and mucosal homeostasis. Our study highlights the novel roles of tRNA-Q modification in maintaining mucosal barriers and innate immunity in intestinal health.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>What is already known about this subject?<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Eukaryotes acquire queuine (q), also known as Vitamin Q, as a micronutrient factor from intestinal microbiota or from diet.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Vitamin Q is needed for queuosine (Q) modification of tRNAs for the protein translation rate and fidelity.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Queuine tRNA-ribosyltransferase catalytic subunit 1 (QTRT1) is reduced in human IBD.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>However, health consequences of disturbed availability of queuine and altered Q-tRNA modification in digestive diseases remain to be investigated.<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>What are the new findings?<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>QTRT1 deficiency leads to altered microbiome and reduced Vitamin Q-associated bacteria in human IBD and a QTRT1 KO animal model.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>QTRT1 protects the host against losing intestinal integrity during inflammation.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>QTRT1 localizes in mitochondria and plays novel functions by maintaining intestinal mitochondrial function. QTRT1 loss impacts tRNA modification in the intestine, linking to mitochondrial integrity and mucosal homeostasis.<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Human IBD showed reduced levels of QTRT1 and dysfunctional mitochondria. Restoring mitochondrial function leads to enhanced QTRT1.<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>How might it impact on clinical practice in the foreseeable future?<\/jats:title>\n                  <jats:p>Targeting tRNA-Q modification in enhancing mitochondrial function will be a novel method to maintain intestinal health.<\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/2025.11.27.690007","type":"posted-content","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T12:16:18Z","timestamp":1764591378000},"source":"Crossref","is-referenced-by-count":1,"title":["Disrupted tRNA modification leads to intestinal mitochondrial dysfunction and microbial dysbiosis"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0528-4072","authenticated-orcid":false,"given":"Di","family":"Ran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongguo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0267-7106","authenticated-orcid":false,"given":"Yueqing","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinglin","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7465-3133","authenticated-orcid":false,"given":"Jun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025120205301420000_2025.11.27.690007v1.1","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1016\/j.jcmgh.2023.02.006","article-title":"Disruption to tRNA Modification by Queuine Contributes to Inflammatory Bowel Disease","volume":"15","year":"2023","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"2025120205301420000_2025.11.27.690007v1.2","doi-asserted-by":"publisher","DOI":"10.3390\/nu7042897"},{"key":"2025120205301420000_2025.11.27.690007v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad667"},{"key":"2025120205301420000_2025.11.27.690007v1.4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1002\/fft2.307","article-title":"Synthesis of queuine by colonic gut microbiome via cross-feeding","volume":"5","year":"2024","journal-title":"Food Frontiers"},{"key":"2025120205301420000_2025.11.27.690007v1.5","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.002477"},{"key":"2025120205301420000_2025.11.27.690007v1.6","doi-asserted-by":"crossref","unstructured":"Alula KM , Dowdell AS , LeBere B , et al. 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