{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T10:46:09Z","timestamp":1785408369329,"version":"3.56.0"},"posted":{"date-parts":[[2025,12,1]]},"group-title":"Cell Biology","reference-count":26,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R01AR056702"],"award-info":[{"award-number":["R01AR056702"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"publisher"}]},{"award":["P30AR069655"],"award-info":[{"award-number":["P30AR069655"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2025,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Lymphatic function is critical for maintaining fluid homeostasis and immune surveillance in synovial tissues, and its dysfunction is implicated in the progression of rheumatoid arthritis and osteoarthritis. Zbtb16, a transcription factor involved in cellular differentiation that is downregulated in mice with inflammatory arthritis and lymphatic defects, has been linked to skeletal development and immune regulation, but its direct role in lymphatic function remains unexplored. To investigate this, we generated Zbtb16\n                  <jats:sup>fl\/fl<\/jats:sup>\n                  mice for conditional-inducible loss-of-function experiments. Initial validation studies demonstrated that CMV-Cre x Zbtb16\n                  <jats:sup>fl\/fl<\/jats:sup>\n                  mice display the same skeletal phenotypes as previously reported Zbtb16\n                  <jats:sup>-\/-<\/jats:sup>\n                  global knockout mice. To achieve telocyte-specific deletion, Efhd1-CreERT2 x Zbtb16\n                  <jats:sup>fl\/fl<\/jats:sup>\n                  mice were treated with tamoxifen, and successful recombination was verified by PCR analysis of genomic DNA from popliteal lymphatic vessel tissue. Functional assessment using NIR-ICG imaging demonstrated that Zbtb16 deletion in telocytes partially impaired lymphatic clearance, suggesting a role in maintaining lymphatic function during homeostasis. These findings demonstrate the utility of Zbtb16\n                  <jats:sup>fl\/fl<\/jats:sup>\n                  mice for their intended purpose and support future studies on the role of Zbtb16 within the synovial lymphatic system.\n                <\/jats:p>","DOI":"10.1101\/2025.11.28.691161","type":"posted-content","created":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T12:10:39Z","timestamp":1764591039000},"source":"Crossref","is-referenced-by-count":0,"title":["Assessment of the Role of Telocyte Zbtb16 in Lymphatic Drainage using Efhd1-CreERT2 x Zbtb16\n                  <sup>flox\/flox<\/sup>\n                  Mice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5524-4772","authenticated-orcid":false,"given":"Yue","family":"Peng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4854-9698","authenticated-orcid":false,"given":"Edward M.","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2025120207300667000_2025.11.28.691161v1.1","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1002\/j.1460-2075.1993.tb05757.x","article-title":"Fusion between a novel Kr\u00fcppel-like zinc finger gene and the retinoic acid receptor-alpha locus due to a variant t(11;17) translocation associated with acute promyelocytic leukaemia","volume":"12","year":"1993","journal-title":"Embo j"},{"key":"2025120207300667000_2025.11.28.691161v1.2","unstructured":"Y. Peng et al., The Function of Efhd1 (+) Telocytes in the Synovial Lymphatic System and Inflammatory-Erosive Arthritis. bioRxiv, (2025)."},{"key":"2025120207300667000_2025.11.28.691161v1.3","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.5.14829"},{"key":"2025120207300667000_2025.11.28.691161v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00289-7"},{"key":"2025120207300667000_2025.11.28.691161v1.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12123"},{"key":"2025120207300667000_2025.11.28.691161v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/35895"},{"key":"2025120207300667000_2025.11.28.691161v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202043"},{"key":"2025120207300667000_2025.11.28.691161v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.013"},{"key":"2025120207300667000_2025.11.28.691161v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/ng1366"},{"key":"2025120207300667000_2025.11.28.691161v1.10","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1788109"},{"key":"2025120207300667000_2025.11.28.691161v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/76014"},{"key":"2025120207300667000_2025.11.28.691161v1.12","doi-asserted-by":"crossref","unstructured":"S. Agrawal Singh et al., PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells. Elife 8, (2019).","DOI":"10.7554\/eLife.40364"},{"key":"2025120207300667000_2025.11.28.691161v1.13","doi-asserted-by":"crossref","unstructured":"B. A. Suliman , D. Xu , B. R. Williams , The promyelocytic leukemia zinc finger protein: two decades of molecular oncology. Front Oncol 2, 74 (2012).","DOI":"10.3389\/fonc.2012.00074"},{"key":"2025120207300667000_2025.11.28.691161v1.14","doi-asserted-by":"crossref","unstructured":"H. Liu et al., Lymphatic-immune interactions in the musculoskeletal system. Front. 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Kenney et al., A high-throughput semi-automated bone segmentation workflow for murine hindpaw micro-CT datasets. Bone Reports 16, 101167 (2022).","DOI":"10.1016\/j.bonr.2022.101167"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/2025.11.28.691161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T10:06:52Z","timestamp":1768471612000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.1101\/2025.11.28.691161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,1]]},"references-count":26,"URL":"https:\/\/doi.org\/10.1101\/2025.11.28.691161","relation":{},"subject":[],"published":{"date-parts":[[2025,12,1]]},"subtype":"preprint"}}