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The base at the polymorphic site is identified by the mass added onto the primer. Heterozygous targets produce two mass-resolved species that represent the addition of both bases complementary to those at the polymorphic site. The assay is suitable for double-stranded PCR products without purification or strand separation. More than one primer can be simultaneously extended and then mass-analyzed. The mass spectrometric method thus shows promise for high-volume diagnostic or genotyping applications.<\/jats:p>","DOI":"10.1101\/gr.7.4.378","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:25:06Z","timestamp":1493771106000},"page":"378-388","source":"Crossref","is-referenced-by-count":180,"title":["Single-Nucleotide Polymorphism Identification Assays Using a Thermostable DNA Polymerase and Delayed Extraction MALDI-TOF Mass\u2009Spectrometry"],"prefix":"10.1101","volume":"7","author":[{"given":"Lawrence A.","family":"Haff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Igor P.","family":"Smirnov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1997,4,1]]},"reference":[{"key":"2021111810264193000_7.4.378.1","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/343220a0","article-title":"PCR test for cystic fibrosis deletion.","volume":"343","author":"Ballabio","year":"1990","journal-title":"Nature"},{"key":"2021111810264193000_7.4.378.2","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1056\/NEJM198703123161103","article-title":"Rapid prenatal diagnosis of sickle cell anemia by a new method of DNA analysis.","volume":"316","author":"Embury","year":"1987","journal-title":"New Engl. 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