{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:29:17Z","timestamp":1792639757133,"version":"4.5.0"},"reference-count":64,"publisher":"Cold Spring Harbor Laboratory","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:p>We have studied the initial development of pluripotent gut endoderm to hepatocytes using a tissue explant system from mouse embryos. We not only find cellular interactions that specify hepatic differentiation but also those that block hepatogenesis in regions of the endoderm that normally give rise to other tissues. The results implicate both positive and negative signaling in early hepatic specification. In vivo footprinting of the albumin enhancer in precursor gut endoderm shows that the transcriptionally silent but potentially active chromatin is characterized by occupancy of an HNF-3 site. Upon hepatic specification, a host of other factors bind nearby sites as the gene becomes active. Genes in pluripotent cells therefore may be marked for potential expression by entry points in chromatin, where additional factors bind during cell type specification. The findings also provide insight into the evolutionary origin of different endodermal cell types.<\/jats:p>","DOI":"10.1101\/gad.10.13.1670","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"1670-1682","source":"Crossref","is-referenced-by-count":523,"title":["Hepatic specification of the gut endoderm in vitro: cell signaling and transcriptional control."],"prefix":"10.1101","volume":"10","author":[{"given":"R","family":"Gualdi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Bossard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Hamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J R","family":"Coleman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K S","family":"Zaret","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[1996,7,1]]},"reference":[{"key":"2021111418382093000_10.13.1670.1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90522-3"},{"key":"2021111418382093000_10.13.1670.2","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1242\/dev.119.4.1301","article-title":"The formation and maintenance of the definitive endoderm lineage in the mouse: Involvement of HNF3\/fork head proteins.","volume":"119","year":"1993","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.3","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90611-8"},{"key":"2021111418382093000_10.13.1670.4","doi-asserted-by":"crossref","first-page":"3617","DOI":"10.1002\/j.1460-2075.1989.tb08535.x","article-title":"Differential expression of myogenic determination genes in muscle cells: Possible auto-activation by the Myf gene products.","volume":"8","year":"1989","journal-title":"EMBO J."},{"key":"2021111418382093000_10.13.1670.5","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1242\/dev.113.1.217","article-title":"Hepatocyte differentiation initiates during endodermal-mesenchymal interactions prior to liver formation.","volume":"113","year":"1991","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.6","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.20.2466"},{"key":"2021111418382093000_10.13.1670.7","doi-asserted-by":"publisher","DOI":"10.1038\/364412a0"},{"key":"2021111418382093000_10.13.1670.8","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90585-X"},{"key":"2021111418382093000_10.13.1670.9","first-page":"4405","article-title":"The extracellular matrix coordinately modulates liver transcription factors and hepatocyte morphology.","volume":"11","year":"1991","journal-title":"Mol. Cell. Biol."},{"key":"2021111418382093000_10.13.1670.10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7598"},{"key":"2021111418382093000_10.13.1670.11","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.5.628"},{"key":"2021111418382093000_10.13.1670.12","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.14.1654"},{"key":"2021111418382093000_10.13.1670.13","first-page":"111","article-title":"Hepatic induction in the avian embryo: Specificity of reactive endoderm and inductive mesoderm.","volume":"63","year":"1981","journal-title":"J. Embryol. Exp. Morphol"},{"key":"2021111418382093000_10.13.1670.14","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1242\/dev.121.8.2439","article-title":"Initiation of cardiac differentiation occurs in the absence of anterior endoderm.","volume":"121","year":"1995","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.7.1749"},{"key":"2021111418382093000_10.13.1670.16","unstructured":"Gilbert, S.F. 1994. Developmental biology. Sinauer Associates, Saunderland, MA."},{"key":"2021111418382093000_10.13.1670.17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.3.1128"},{"key":"2021111418382093000_10.13.1670.18","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(74)90126-3"},{"key":"2021111418382093000_10.13.1670.19","doi-asserted-by":"publisher","DOI":"10.1038\/336772a0"},{"key":"2021111418382093000_10.13.1670.20","doi-asserted-by":"publisher","DOI":"10.1038\/348169a0"},{"key":"2021111418382093000_10.13.1670.21","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6039(80)90026-3"},{"key":"2021111418382093000_10.13.1670.22","first-page":"1531","article-title":"Suppression of albumin enhancer activity by H-ras and AP-1 in hepatocyte cell lines.","volume":"14","year":"1994","journal-title":"Mol. Cell Biol."},{"key":"2021111418382093000_10.13.1670.23","first-page":"2401","article-title":"Modulation of liver-specific transcription by interactions between hepatocyte nuclear factor 3 and nuclear factor 1 binding DNA in close apposition.","volume":"13","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"2021111418382093000_10.13.1670.24","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.4.957"},{"key":"2021111418382093000_10.13.1670.25","first-page":"416","article-title":"Hepatocyte nuclear factor 3A belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head.","volume":"56","year":"1991","journal-title":"Genes & Dev."},{"key":"2021111418382093000_10.13.1670.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10421"},{"key":"2021111418382093000_10.13.1670.27","first-page":"543","article-title":"Etude exp\u00e9rimentale de l'organogen\u00e8se du tube digestif et du foie chez l'embryon de poulet.","volume":"98","year":"1964","journal-title":"Bull. Biol. Fr. Belg."},{"key":"2021111418382093000_10.13.1670.28","first-page":"427","article-title":"An experimental analysis of liver development.","volume":"53","year":"1975","journal-title":"Med. Biol."},{"key":"2021111418382093000_10.13.1670.29","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90583-6"},{"key":"2021111418382093000_10.13.1670.30","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.5.528"},{"key":"2021111418382093000_10.13.1670.31","first-page":"773","article-title":"Extracellular signals that regulate liver transcription factors during hepatic differentiation in vitro.","volume":"11","year":"1991","journal-title":"Mol. Cell. Biol."},{"key":"2021111418382093000_10.13.1670.32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.5.2133"},{"key":"2021111418382093000_10.13.1670.33","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80079-T"},{"key":"2021111418382093000_10.13.1670.34","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1242\/dev.119.3.567","article-title":"Postimplantation expression patterns indicate a role for the mouse fork head\/HNF-3\u03b1, \u03b2, and \u03b3 genes in determination of the definitive endoderm, chordamesoderm and neuroectoderm.","volume":"119","year":"1993","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.35","doi-asserted-by":"publisher","DOI":"10.1126\/science.2814500"},{"key":"2021111418382093000_10.13.1670.36","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1.3.268"},{"key":"2021111418382093000_10.13.1670.37","doi-asserted-by":"publisher","DOI":"10.1126\/science.3041594"},{"key":"2021111418382093000_10.13.1670.38","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.12b.2050"},{"key":"2021111418382093000_10.13.1670.39","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90370-E"},{"key":"2021111418382093000_10.13.1670.40","unstructured":"Rugh, R. 1968. The mouse: Its reproduction and development. Burgess Publishing Company, Minneapolis, MN."},{"key":"2021111418382093000_10.13.1670.41","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1242\/dev.118.1.47","article-title":"Differential expression of multiple fork head related genes during gastrulation and pattern formation in the mouse embryo.","volume":"118","year":"1993","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.42","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1242\/dev.104.1.155","article-title":"Transcripts of \u03b1-cardiac and \u03b1-skeletal actins are early markers for myogenesis in the mouse embryo.","volume":"104","year":"1988","journal-title":"Development"},{"key":"2021111418382093000_10.13.1670.43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1990.tb00462.x"},{"key":"2021111418382093000_10.13.1670.44","first-page":"139","article-title":"Enzymo- and immunocytochemical analyses of the differentiation of liver cells in the prenatal mouse.","volume":"62","year":"1981","journal-title":"J. Embryol. Exp. Morphol."},{"key":"2021111418382093000_10.13.1670.45","first-page":"2611","article-title":"Cell lineages and oval cell progenitors in rat liver development.","volume":"51","year":"1991","journal-title":"Cancer Res."},{"key":"2021111418382093000_10.13.1670.46","doi-asserted-by":"crossref","unstructured":"Slack, J.M.W. 1991. From egg to embryo: Regional specification in early development. Cambridge University Press, Cambridge, UK.","DOI":"10.1017\/CBO9780511525322"},{"key":"2021111418382093000_10.13.1670.47","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.12b.2353"},{"key":"2021111418382093000_10.13.1670.48","doi-asserted-by":"publisher","DOI":"10.1038\/335551a0"},{"key":"2021111418382093000_10.13.1670.49","doi-asserted-by":"crossref","unstructured":"Theiler, K. 1989. The house mouse. Springer-Verlag, New York, NY.","DOI":"10.1007\/978-3-642-88418-4"},{"key":"2021111418382093000_10.13.1670.50","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.17.5254"},{"key":"2021111418382093000_10.13.1670.51","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90006-X"},{"key":"2021111418382093000_10.13.1670.52","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.4.915"},{"key":"2021111418382093000_10.13.1670.53","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90133-5"},{"key":"2021111418382093000_10.13.1670.54","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90523-1"},{"key":"2021111418382093000_10.13.1670.55","unstructured":"Wessells, N.K. 1977. Tissue interaction and development. Benjamin, Menlo Park, NY."},{"key":"2021111418382093000_10.13.1670.56","doi-asserted-by":"crossref","unstructured":"Wilkinson, D.G. 1993. In situ hybridization. In Essential developmental biology: A practical approach (ed. CD. Stern and P.W.H. Holland), pp. 257\u2013274, IRL Press, Oxford, UK.","DOI":"10.1093\/oso\/9780199634231.003.0026"},{"key":"2021111418382093000_10.13.1670.57","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90583-7"},{"key":"2021111418382093000_10.13.1670.58","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.3.881"},{"key":"2021111418382093000_10.13.1670.59","doi-asserted-by":"publisher","DOI":"10.1006\/scel.1995.0029"},{"key":"2021111418382093000_10.13.1670.60","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.001311"},{"key":"2021111418382093000_10.13.1670.61","first-page":"342","article-title":"Primer extension analysis of RNA.","volume":"2","year":"1993","journal-title":"Methods Mol. Genet."},{"key":"2021111418382093000_10.13.1670.62","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1995.0014"},{"key":"2021111418382093000_10.13.1670.63","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5469"},{"key":"2021111418382093000_10.13.1670.64","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1002\/j.1460-2075.1993.tb05685.x","article-title":"The expression pattern of a Drosophila homolog to the mouse transcription factor HNF-3 suggests a determinative role in gut formation.","volume":"12","year":"1993","journal-title":"EMBO J."}],"container-title":["Genes &amp; Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/gad.10.13.1670","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T21:29:43Z","timestamp":1707946183000},"score":1,"resource":{"primary":{"URL":"http:\/\/genesdev.cshlp.org\/lookup\/doi\/10.1101\/gad.10.13.1670"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7,1]]},"references-count":64,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1996,7,1]]}},"alternative-id":["10.1101\/gad.10.13.1670"],"URL":"https:\/\/doi.org\/10.1101\/gad.10.13.1670","relation":{},"ISSN":["0890-9369","1549-5477"],"issn-type":[{"value":"0890-9369","type":"print"},{"value":"1549-5477","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7,1]]}}}