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Others have shown that exposure of scid mice to genotoxic agents such as gamma-irradiation rescues rearrangement at the T-cell receptor (TCR) beta locus and promotes thymocyte development. Here we demonstrate that irradiation rescues rearrangements at multiple TCR loci, suggesting a general effect on the recombination mechanism. Furthermore, our data show that p53 is required for irradiation-mediated rescue of both thymocyte development and V(D)J recombination. We also find that thymocyte proliferation and differentiation in the absence of DNA damage do not require p53 and are not sufficient to rescue V(D)J recombination. These results suggest that exposure to ionizing radiation facilitates a partial bypass of the scid defect, perhaps by inducing p53-dependent DNA damage response pathways.<\/jats:p>","DOI":"10.1101\/gad.10.5.553","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"553-565","source":"Crossref","is-referenced-by-count":80,"title":["p53 is required for both radiation-induced differentiation and rescue of V(D)J rearrangement in scid mouse thymocytes."],"prefix":"10.1101","volume":"10","author":[{"given":"M A","family":"Bogue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Aguilar-Cordova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L A","family":"Donehower","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D B","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[1996,3,1]]},"reference":[{"key":"2021111418284861000_10.5.553.1","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.4049\/jimmunol.146.4.1348","article-title":"V\u03b33 T cell receptor rearrangement and expression in the adult thymus.","volume":"146","year":"1991","journal-title":"J. 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