{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:02:53Z","timestamp":1779343373420,"version":"3.51.4"},"reference-count":45,"publisher":"Cold Spring Harbor Laboratory","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:p>The Src tyrosine kinase has been implicated in a wide variety of signal transduction pathways, yet despite the nearly ubiquitous expression of c-<jats:italic>src, src<\/jats:italic>\u2212\/\u2212 mice show only one major phenotype\u2014osteopetrosis caused by an intrinsic defect in osteoclasts, the cells responsible for resorbing bone. To explore further the role of Src both in osteoclasts and other cell types, we have generated transgenic mice that express the wild-type and mutated versions of the chicken c-<jats:italic>src<\/jats:italic> proto-oncogene from the promoter of <jats:italic>tartrate resistant acid phosphatase<\/jats:italic> (<jats:italic>TRAP<\/jats:italic>), a gene that is expressed highly in osteoclasts. We demonstrate here that expression of a wild-type transgene in only a limited number of tissues can fully rescue the <jats:italic>src<\/jats:italic>\u2212\/\u2212 phenotype. Surprisingly, expression of kinase-defective alleles of c-<jats:italic>src<\/jats:italic>also reduces osteopetrosis in <jats:italic>src<\/jats:italic>\u2212\/\u2212 animals and partially rescues a defect in cytoskeletal organization observed in <jats:italic>src<\/jats:italic>\u2212\/\u2212 osteoclasts. These results suggest that there are essential kinase-independent functions for Src in vivo. Biochemical examination of osteoclasts from these mice suggest that Src may function in part by recruiting or activating other tyrosine kinases.<\/jats:p>","DOI":"10.1101\/gad.11.21.2835","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T22:15:26Z","timestamp":1203545726000},"page":"2835-2844","source":"Crossref","is-referenced-by-count":236,"title":["Rescue of osteoclast function by transgenic expression of kinase-deficient Src in<i>src<\/i>\u2212\/\u2212 mutant\u2009mice"],"prefix":"10.1101","volume":"11","author":[{"given":"Pamela L.","family":"Schwartzberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lianping","family":"Xing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oskar","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clifford A.","family":"Lowell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa","family":"Garrett","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan F.","family":"Boyce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold E.","family":"Varmus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1997,11,1]]},"reference":[{"key":"2021111418481252000_11.21.2835.1","doi-asserted-by":"crossref","unstructured":"Baron R. 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