{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:08:42Z","timestamp":1791050922441,"version":"4.1.0"},"reference-count":64,"publisher":"Cold Spring Harbor Laboratory","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>To learn about the acquisition of neural fate by ectodermal cells, we have analyzed a very early sign of neural commitment in<jats:italic>Drosophila,<\/jats:italic> namely the specific accumulation of<jats:italic>achaete\u2013scute<\/jats:italic> complex (AS-C) proneural proteins in the cell that becomes a sensory organ mother cell (SMC). We have characterized an AS-C enhancer that directs expression specifically in SMCs. This enhancer promotes Scute protein accumulation in these cells, an event essential for sensory organ development in the absence of other AS-C genes. Interspecific sequence comparisons and site-directed mutagenesis show the presence of several conserved motifs necessary for enhancer action, some of them binding sites for proneural proteins. These and other data indicate that the enhancer mediates <jats:italic>scute<\/jats:italic>self-stimulation, although only in the presence of additional activating factors, which most likely interact with conserved motifs reminiscent of NF-\u03baB-binding sites. Cells neighboring the SMC do not acquire the neural fate because the <jats:italic>Notch<\/jats:italic> signaling pathway effectors, the <jats:italic>Enhancer of split<\/jats:italic> bHLH proteins, block this proneural gene self-stimulatory loop, possibly by antagonizing the action on the enhancer of the NF-\u03baB-like factors or the proneural proteins. These data suggest a mechanism for SMC committment.<\/jats:p>","DOI":"10.1101\/gad.12.13.2036","type":"journal-article","created":{"date-parts":[[2008,2,20]],"date-time":"2008-02-20T22:52:17Z","timestamp":1203547937000},"page":"2036-2047","source":"Crossref","is-referenced-by-count":147,"title":["Proneural gene self-stimulation in neural precursors: an essential mechanism for sense organ development that is regulated by<i>Notch<\/i>\u2009signaling"],"prefix":"10.1101","volume":"12","author":[{"given":"Joaquim","family":"Cul\u00ed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Modolell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"2021111418545521000_12.13.2036.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.24.13099"},{"key":"2021111418545521000_12.13.2036.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90539-1"},{"key":"2021111418545521000_12.13.2036.3","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(91)90220-K"},{"key":"2021111418545521000_12.13.2036.4","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2609"},{"key":"2021111418545521000_12.13.2036.5","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1995.8033"},{"key":"2021111418545521000_12.13.2036.6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/dev.119.1.1","article-title":"asense is a Drosophila neural precursor gene and is capable of initiating sense organ formation.","volume":"119","author":"Brand","year":"1993","journal-title":"Development"},{"key":"2021111418545521000_12.13.2036.7","doi-asserted-by":"crossref","first-page":"2965","DOI":"10.1002\/j.1460-2075.1991.tb07847.x","article-title":"Transcriptional activation by heterodimers of the achaete-scute and daughterless gene products of Drosophila.","volume":"10","author":"Cabrera","year":"1991","journal-title":"EMBO J."},{"key":"2021111418545521000_12.13.2036.8","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(92)90234-U"},{"key":"2021111418545521000_12.13.2036.9","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90147-3"},{"key":"2021111418545521000_12.13.2036.10","doi-asserted-by":"crossref","first-page":"3385","DOI":"10.1002\/j.1460-2075.1992.tb05417.x","article-title":"The extramacrochaetae gene provides information for sensory organ patterning.","volume":"11","author":"Cubas","year":"1992","journal-title":"EMBO J."},{"key":"2021111418545521000_12.13.2036.11","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.6.996"},{"key":"2021111418545521000_12.13.2036.12","doi-asserted-by":"crossref","first-page":"6923","DOI":"10.1128\/MCB.15.12.6923","article-title":"Specificity for the Hairy\/Enhancer of split basic helix-loop-helix (bHLH) proteins map outside the bHLH domain and suggests two separable models of transcriptional repression.","volume":"15","author":"Dawson","year":"1995","journal-title":"Mol. 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