{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:47:10Z","timestamp":1792622830676,"version":"4.5.0"},"reference-count":61,"publisher":"Cold Spring Harbor Laboratory","issue":"21","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[2000,11,1]]},"abstract":"<jats:p>\n                    The Pax6 transcription factor plays a key role in ocular development of vertebrates and invertebrates. Homozygosity of the\n                    <jats:italic>Pax6<\/jats:italic>\n                    null mutation in human and mice results in arrest of optic vesicle development and failure to initiate lens formation. This phenotype obscures the understanding of autonomous function of Pax6 in these tissue components and during later developmental stages. We employed the Cre\/\n                    <jats:italic>loxP<\/jats:italic>\n                    approach to inactivate\n                    <jats:italic>Pax6<\/jats:italic>\n                    specifically in the eye surface ectoderm concomitantly with lens induction. Although lens induction occurred in the mutant, as indicated by\n                    <jats:italic>Sox2<\/jats:italic>\n                    up-regulation in the surface ectoderm, further development of the lens was arrested. Hence, Pax6 activity was found to be essential in the specified ectoderm for lens placode formation. Furthermore, this mutant model allowed us for the first time to address in vivo the development of a completely normal retina in the absence of early lens structures. Remarkably, several independent, fully differentiated neuroretinas developed in a single optic vesicle in the absence of a lens, demonstrating that the developing lens is not necessary to instruct the differentiation of the neuroretina but is, rather, required for the correct placement of a single retina in the eye.\n                  <\/jats:p>","DOI":"10.1101\/gad.184000","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T20:00:34Z","timestamp":1027713634000},"page":"2701-2711","source":"Crossref","is-referenced-by-count":479,"title":["Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye"],"prefix":"10.1101","volume":"14","author":[{"given":"Ruth","family":"Ashery-Padan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"Marquardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunlei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Gruss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2000,11,1]]},"reference":[{"key":"2021111419241934000_14.21.2701.1","doi-asserted-by":"crossref","first-page":"1425","DOI":"10.1523\/JNEUROSCI.17-04-01425.1997","article-title":"The expression and function of Notch pathway genes in the developing rat eye.","volume":"17","author":"Bao","year":"1997","journal-title":"J. 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Walton S. Cai J. Epstein J. Jepeal L. Mass R.L. (1995) Pax6 gene mutations in aniridia. in Molecular genetics of ocular diseases, ed Wiggs J. 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