{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:37:30Z","timestamp":1791243450587,"version":"4.1.0"},"reference-count":36,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[2001,6,15]]},"abstract":"<jats:p><jats:italic>casanova<\/jats:italic> (<jats:italic>cas<\/jats:italic>) mutant zebrafish embryos lack endoderm and develop cardia bifida. In a substractive screen for Nodal-responsive genes, we isolated an HMG box-containing gene,<jats:italic>10J3<\/jats:italic>, which is expressed in the endoderm. The<jats:italic>cas<\/jats:italic> phenotype is rescued by overexpression of <jats:italic>10J3<\/jats:italic>and can be mimicked by <jats:italic>10J3<\/jats:italic>-directed morpholinos. Furthermore, we identified a mutation within <jats:italic>10J3<\/jats:italic> coding sequence that cosegregates with the <jats:italic>cas<\/jats:italic> phenotype, clearly demonstrating that <jats:italic>cas<\/jats:italic> is encoded by <jats:italic>10J3<\/jats:italic>. Epistasis experiments are consistent with an instructive role for<jats:italic>cas<\/jats:italic> in endoderm formation downstream of Nodal signals and upstream of <jats:italic>sox17<\/jats:italic>. In the absence of <jats:italic>cas<\/jats:italic>activity, endoderm progenitors differentiate into mesodermal derivatives. Thus, <jats:italic>cas<\/jats:italic> is an HMG box-containing gene involved in the fate decision between endoderm and mesoderm that acts downstream of Nodal signals.<\/jats:p>","DOI":"10.1101\/gad.196901","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T23:59:15Z","timestamp":1027727955000},"page":"1487-1492","source":"Crossref","is-referenced-by-count":173,"title":["A crucial component of the endoderm formation pathway, CASANOVA, is encoded by a novel <i>sox<\/i>-related 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