{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:07:05Z","timestamp":1791173225285,"version":"4.1.0"},"reference-count":46,"publisher":"Cold Spring Harbor Laboratory","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"published-print":{"date-parts":[[2001,9,1]]},"abstract":"<jats:p>Conformational changes in sigma 54 (\u03c2<jats:sup>54<\/jats:sup>) and \u03c2<jats:sup>54<\/jats:sup>-holoenzyme depend on nucleotide hydrolysis by an activator. We now show that \u03c2<jats:sup>54<\/jats:sup> and its holoenzyme bind to the central ATP-hydrolyzing domains of the transcriptional activators PspF and NifA in the presence of ADP\u2013aluminum fluoride, an analog of ATP in the transition state for hydrolysis. Direct binding of \u03c2<jats:sup>54<\/jats:sup> Region I to activator in the presence of ADP\u2013aluminum fluoride was shown and inferred from in vivo suppression genetics. Energy transduction appears to occur through activator contacts to \u03c2<jats:sup>54<\/jats:sup> Region I. ADP\u2013aluminum fluoride-dependent interactions and consideration of other AAA+ proteins provide insight into activator mechanochemical action.<\/jats:p>","DOI":"10.1101\/gad.205501","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T00:00:34Z","timestamp":1027728034000},"page":"2282-2294","source":"Crossref","is-referenced-by-count":117,"title":["Binding of transcriptional activators to sigma 54 in the presence of the transition state analog ADP\u2013aluminum fluoride: insights into activator mechanochemical action"],"prefix":"10.1101","volume":"15","author":[{"given":"Matthew","family":"Chaney","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Grande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siva R.","family":"Wigneshweraraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Cannon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Casaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria-Trinidad","family":"Gallegos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorg","family":"Schumacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Elderkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angel Ernesto","family":"Dago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique","family":"Morett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Buck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2001,9,1]]},"reference":[{"key":"2021111507421572000_15.17.2282.1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.15.4129-4136.2000"},{"key":"2021111507421572000_15.17.2282.2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3161"},{"key":"2021111507421572000_15.17.2282.3","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0260"},{"key":"2021111507421572000_15.17.2282.4","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.3.357"},{"key":"2021111507421572000_15.17.2282.5","doi-asserted-by":"publisher","DOI":"10.1038\/76830"},{"key":"2021111507421572000_15.17.2282.6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007779200"},{"key":"2021111507421572000_15.17.2282.7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.22.12145"},{"key":"2021111507421572000_15.17.2282.8","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2328"},{"key":"2021111507421572000_15.17.2282.9","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3076"},{"key":"2021111507421572000_15.17.2282.10","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01566.x"},{"key":"2021111507421572000_15.17.2282.11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002253200"},{"key":"2021111507421572000_15.17.2282.12","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1002\/j.1460-2075.1986.tb04230.x","article-title":"Sequence and domain relationships of NtrC and NifA from Klebsiella pneumoniae: Homologies to other regulatory proteins.","volume":"5","author":"Drummond","year":"1986","journal-title":"EMBO J."},{"key":"2021111507421572000_15.17.2282.13","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.2.311-319.2000"},{"key":"2021111507421572000_15.17.2282.14","unstructured":"Fersht A. (1998) Structure and mechanism in protein science. (Freeman, New York)."},{"key":"2021111507421572000_15.17.2282.15","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2704"},{"key":"2021111507421572000_15.17.2282.16","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3608"},{"key":"2021111507421572000_15.17.2282.17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25285"},{"key":"2021111507421572000_15.17.2282.18","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(98)80038-9"},{"key":"2021111507421572000_15.17.2282.19","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00772.x"},{"key":"2021111507421572000_15.17.2282.20","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3232"},{"key":"2021111507421572000_15.17.2282.21","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.13.3736"},{"key":"2021111507421572000_15.17.2282.22","doi-asserted-by":"publisher","DOI":"10.1101\/gad.794800"},{"key":"2021111507421572000_15.17.2282.23","doi-asserted-by":"publisher","DOI":"10.1007\/s000180050421"},{"key":"2021111507421572000_15.17.2282.24","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2263"},{"key":"2021111507421572000_15.17.2282.25","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.22.6503-6508.2000"},{"key":"2021111507421572000_15.17.2282.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.21.9702"},{"key":"2021111507421572000_15.17.2282.27","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.18.5188-5195.2000"},{"key":"2021111507421572000_15.17.2282.28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.21.7607"},{"key":"2021111507421572000_15.17.2282.29","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0399"},{"key":"2021111507421572000_15.17.2282.30","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006593"},{"key":"2021111507421572000_15.17.2282.31","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1359-1368.2001"},{"key":"2021111507421572000_15.17.2282.32","doi-asserted-by":"crossref","first-page":"6067","DOI":"10.1128\/jb.175.19.6067-6074.1993","article-title":"The \u03c254 bacterial enhancer-binding protein family: Mechanism of action and phylogenetic relationship of their functional domains.","volume":"175","author":"Morett","year":"1993","journal-title":"J. Bacteriol."},{"key":"2021111507421572000_15.17.2282.33","doi-asserted-by":"publisher","DOI":"10.1021\/bi00146a023"},{"key":"2021111507421572000_15.17.2282.34","doi-asserted-by":"publisher","DOI":"10.1101\/gr.9.1.27"},{"key":"2021111507421572000_15.17.2282.35","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.22.4419"},{"key":"2021111507421572000_15.17.2282.36","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.1998.63.157"},{"key":"2021111507421572000_15.17.2282.37","doi-asserted-by":"crossref","first-page":"5619","DOI":"10.1128\/JB.180.21.5619-5625.1998","article-title":"Identification of an N-terminal region of \u03c254 required for enhancer responsiveness.","volume":"180","author":"Syed","year":"1998","journal-title":"J. Bacteriol."},{"key":"2021111507421572000_15.17.2282.38","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.150.1.F13"},{"key":"2021111507421572000_15.17.2282.39","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5238.992"},{"key":"2021111507421572000_15.17.2282.40","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5851955.x"},{"key":"2021111507421572000_15.17.2282.41","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.16.2042"},{"key":"2021111507421572000_15.17.2282.42","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.3038"},{"key":"2021111507421572000_15.17.2282.43","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4393"},{"key":"2021111507421572000_15.17.2282.44","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00355-1"},{"key":"2021111507421572000_15.17.2282.45","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5306.1658"},{"key":"2021111507421572000_15.17.2282.46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13142"}],"container-title":["Genes &amp; Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/gad.205501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,15]],"date-time":"2021-11-15T15:44:10Z","timestamp":1636991050000},"score":1,"resource":{"primary":{"URL":"http:\/\/genesdev.cshlp.org\/lookup\/doi\/10.1101\/gad.205501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9,1]]},"references-count":46,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2001,9,1]]}},"alternative-id":["10.1101\/gad.205501"],"URL":"https:\/\/doi.org\/10.1101\/gad.205501","relation":{},"ISSN":["0890-9369","1549-5477"],"issn-type":[{"value":"0890-9369","type":"print"},{"value":"1549-5477","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,9,1]]}}}