{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:57:46Z","timestamp":1785772666722,"version":"3.56.0"},"reference-count":62,"publisher":"Cold Spring Harbor Laboratory","issue":"15-16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"accepted":{"date-parts":[[2026,3,25]]},"published-print":{"date-parts":[[2026,8,1]]},"abstract":"<jats:p>\n                    <jats:italic>Su(var)<\/jats:italic>\n                    genes regulate the formation and maintenance of heterochromatin in\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    , yet how factors associated with active chromatin influence heterochromatin stability remains poorly understood. Through a new genetic\n                    <jats:italic>Su(var)<\/jats:italic>\n                    screen, we identified two strong suppressors of position effect variegation that remain epistatic to increased\n                    <jats:italic>Su(var)3-9<\/jats:italic>\n                    dosage in the\n                    <jats:italic>In(1)w<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>m4h<\/jats:italic>\n                    <\/jats:sup>\n                    rearrangement. Both mutations affect the PWWP domain of the transcriptional cofactor\n                    <jats:italic>JASPer<\/jats:italic>\n                    , previously implicated in safeguarding transcription within euchromatin. In nuclear cycle (NC14) mutant embryos carrying these mutations, canonical heterochromatic marks (H3K9me2\/3) are reduced, while activating histone modifications (H3K4me1 and H3K27ac) accumulate at heterochromatin regions. Whereas wild-type JASPer localizes predominantly to H3K36me3-enriched gene bodies, the mutant protein (JASPer\n                    <jats:sup>Su140<\/jats:sup>\n                    ) shows reduced chromatin-association and it is redistributed to\n                    <jats:italic>cis<\/jats:italic>\n                    -regulatory elements. Notably, a subset of developmentally regulated enhancers, including the\n                    <jats:italic>white<\/jats:italic>\n                    enhancer, becomes enriched for JASPer\n                    <jats:sup>Su140<\/jats:sup>\n                    and display elevated enhancer-RNAs transcription at NC14 embryos. Consistent with these molecular changes, mutant embryos exhibit partial decompaction of the heterochromatin domain containing the\n                    <jats:italic>In(1)w<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>m4h<\/jats:italic>\n                    <\/jats:sup>\n                    rearrangement and activation of satellite repeat transcription. Together, our findings identify JASPer as a previously unrecognized Su(var) factor and reveal that proper partitioning between euchromatin and heterochromatin domains is critical for maintaining position effect variegation.\n                  <\/jats:p>","DOI":"10.1101\/gad.353541.125","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:00:21Z","timestamp":1784228421000},"page":"1330-1349","source":"Crossref","is-referenced-by-count":0,"title":["PWWP mutants of the transcriptional cofactor JASPer divert promoter and enhancer activities and suppress position effect variegation in\n                    <i>Drosophila<\/i>"],"prefix":"10.1101","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6097-3670","authenticated-orcid":false,"given":"Derek","family":"Atkinson","sequence":"first","affiliation":[{"name":"Max Planck Institute of Immunology and Epigenetics, Freiburg 79108, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6990-9444","authenticated-orcid":false,"given":"Karolina","family":"Barucka","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Immunology and Epigenetics, Freiburg 79108, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Jerabek","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Immunology and Epigenetics, Freiburg 79108, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5880-8270","authenticated-orcid":false,"given":"Francesco","family":"Cardamone","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Immunology and Epigenetics, Freiburg 79108, Germany;"},{"name":"Faculty of Biology, University of Freiburg, Freiburg 79108, 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