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Here we combine comparative genomics with genetic and single-cell analyses in zebrafish and mice to show that MEIOSIN retains an intrinsic STRA8-independent activity. We identify\n                    <jats:italic>Meiosin<\/jats:italic>\n                    orthologs in zebrafish and hagfish, vertebrate lineages that lack Stra8, and show that these proteins retain the HMG domain but have lost the bHLH domain required for the canonical MEIOSIN\u2013STRA8 interaction. In zebrafish,\n                    <jats:italic>meiosin<\/jats:italic>\n                    is transiently induced at meiotic entry in both sexes, yet its loss selectively disrupts the female germline, causing failure of oogenesis and female-to-male sex reversal. In mice, MEIOSIN lacking the bHLH domain still initiates key features of meiotic entry and partially activates meiotic target genes, although it fails to support full meiotic progression. Together, these findings support a model in which HMG-containing MEIOSIN provides a conserved core activity for meiotic gene activation, whereas STRA8 and the bHLH-mediated MEIOSIN\u2013STRA8 interaction reinforce the efficiency and robustness of this program in lineages that retain the canonical module.\n                  <\/jats:p>","DOI":"10.1101\/gad.353802.126","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:39:10Z","timestamp":1787668750000},"page":"1629-1648","source":"Crossref","is-referenced-by-count":1,"title":["MEIOSIN retains a STRA8-independent activity that contributes to meiotic gene activation across vertebrates"],"prefix":"10.1101","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6724-0875","authenticated-orcid":false,"given":"Ryuki","family":"Shimada","sequence":"first","affiliation":[{"name":"Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 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Kumamoto University, Kumamoto 860-8556, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6537-3284","authenticated-orcid":false,"given":"Toshihiro","family":"Kawasaki","sequence":"additional","affiliation":[{"name":"Model Fish Genetics Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics (NIG), Mishima, Shizuoka 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakie","family":"Iisaka","sequence":"additional","affiliation":[{"name":"Department of Chromosome Biology, Institute of Molecular Embryology and Genetics (IMEG), Kumamoto University, Kumamoto 860-0811, Japan;"},{"name":"Department of Biomedical Science, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Usuki","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei-ichiro","family":"Yasunaga","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayoko","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Tani","sequence":"additional","affiliation":[{"name":"Liaison Laboratory Research Promotion Center, IMEG, Kumamoto University, Kumamoto 860-0811, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Niwa","sequence":"additional","affiliation":[{"name":"Department of Pluripotent Stem Cell Biology, IMEG, Kumamoto University, Kumamoto 860-0811, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1464-8388","authenticated-orcid":false,"given":"Shigehiro","family":"Kuraku","sequence":"additional","affiliation":[{"name":"Molecular Life History Laboratory, NIG, Mishima 411-8540, Japan;"},{"name":"Department of Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Model Fish Genetics Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics (NIG), Mishima, Shizuoka 411-8540, Japan;"},{"name":"Department of Genetics, Sokendai (Graduate University for Advanced Studies), Mishima, Shizuoka 411-8540, 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