{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:50:40Z","timestamp":1791910240433,"version":"4.3.4"},"reference-count":0,"publisher":"Cold Spring Harbor Laboratory","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM134867"],"award-info":[{"award-number":["R35GM134867"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences of the National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM151262"],"award-info":[{"award-number":["R35GM151262"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences of the National Institutes of Health","doi-asserted-by":"publisher","award":["P20GM103474"],"award-info":[{"award-number":["P20GM103474"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Dev."],"accepted":{"date-parts":[[2026,8,6]]},"abstract":"<jats:p>\n                    While Polycomb-based gene silencing relies upon the generation of large, condensed domains of histone H3K27 trimethylation, the mechanisms forming and maintaining these domains are incompletely understood. Several previously identified mechanisms concentrate PRC2 and its H3K27me3 activity, including allosteric activation, binding to H2Aub, and concentration into PcG bodies through weak multivalent interactions with other Polycomb group proteins. Here, using the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    nurse cell system to study silencing initiation in developing tissue, we discover a new mechanism. The scaffolding protein Sex comb on midleg (Scm) first polymerizes into PcG bodies independently of PRC1 and PRC2. These Scm-containing bodies then recruit PRC2 through a direct, structured interaction between the Scm\u2013Zf-FCS domain and the Pcl atypical Tudor domain. Finally, we show that the Scm\u2013Zf-FCS\/Pcl\u2013Tudor interaction is required for viability and Hox gene regulation during somatic cell development. Our work discovers a new mechanism anchoring PRC2 to PcG bodies that explains how cells can rapidly create and maintain large domains of H3K27me3. It also raises a new possibility that cells could regulate Scm polymerization to determine the size and location of H3K27me3 domains.\n                  <\/jats:p>","DOI":"10.1101\/gad.353999.126","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:15:12Z","timestamp":1790010912000},"source":"Crossref","is-referenced-by-count":1,"title":["<i>Drosophila<\/i>\n                    Scm self-assembles into PcG bodies to recruit PRC2.1 to target genes during Polycomb silencing initiation"],"prefix":"10.1101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5721-4080","authenticated-orcid":false,"given":"David B.","family":"Lyons","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corbin J.","family":"Arbizzani","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5438-0879","authenticated-orcid":false,"given":"Oguz","family":"Kanca","sequence":"additional","affiliation":[{"name":"Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, Texas 77030, USA;"},{"name":"Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0683-8413","authenticated-orcid":false,"given":"Steven Z.","family":"DeLuca","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology, Montana State University, Bozeman, Montana 59717, USA;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2026,9,21]]},"container-title":["Genes &amp; Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/gad.353999.126","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:15:13Z","timestamp":1791908113000},"score":1,"resource":{"primary":{"URL":"http:\/\/genesdev.cshlp.org\/lookup\/doi\/10.1101\/gad.353999.126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,21]]},"references-count":0,"alternative-id":["10.1101\/gad.353999.126"],"URL":"https:\/\/doi.org\/10.1101\/gad.353999.126","relation":{},"ISSN":["0890-9369","1549-5477"],"issn-type":[{"value":"0890-9369","type":"print"},{"value":"1549-5477","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,21]]}}}