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These GATA\/ER chimeric factors were shown to be hormone-inducible trans-activating proteins in transient transfection assays. When stably introduced into primary erythroblasts or conditionally transformed erythroid progenitors cells, exogenous GATA-2\/ER promoted proliferation and inhibited terminal differentiation in an estrogen-dependent manner. These phenotypic effects are specifically attributable to the action of ectopically expressed GATA-2\/ER because erythroblasts expressing exogenous GATA-2 are constitutively arrested in differentiation and because erythroid progenitors expressing either Gal\/ER or GATA-3\/ER do not display a hormone-responsive block in differentiation. Thus, the GATA-2 transcription factor appears to play a role in regulating the self-renewal capacity of early erythroid progenitor cells.<\/jats:p>","DOI":"10.1101\/gad.7.6.1097","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"1097-1109","source":"Crossref","is-referenced-by-count":172,"title":["Ectopic expression of a conditional GATA-2\/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner."],"prefix":"10.1101","volume":"7","author":[{"given":"K","family":"Briegel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K C","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Plank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Beug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J D","family":"Engel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Zenke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[1993,6,1]]},"reference":[{"key":"2021111418091203000_7.6.1097.1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90070-8"},{"key":"2021111418091203000_7.6.1097.2","first-page":"195","article-title":"Erythroblast cell lines transformed by a temperature-sensitive mutant of avian erythroblastosis virus: A model system to study erythro1id differentiation in vitro.","volume":"1","year":"1982","journal-title":"J. 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