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Four scrapie-affected sheep were homozygous for wild-type PrP open reading frames encoding the alternative Gln (Q)-171 allele. Analysis of additional cases revealed that all were Q\/Q-171 homozygotes (n = 31), yielding a probability of 0.000004 that PrP genotype is unrelated to susceptibility. These data imply that homozygosity for Q-171 codons is necessary but not sufficient for the development of natural scrapie, echo reports of recessive manifestation, and parallel over-representation of PRNP codon 129 homozygotes in Creutzfeldt-Jakob disease of humans. Whereas progress has been substantial regarding experimental scrapie in rodents, the occurrence and spread of disease in flocks of sheep has remained enigmatic. Appreciation of the relationship between codon 171 genotype and susceptibility may help define the molecular basis of natural scrapie.<\/jats:p>","DOI":"10.1101\/gad.8.8.959","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:15:46Z","timestamp":1181078146000},"page":"959-969","source":"Crossref","is-referenced-by-count":201,"title":["Homozygosity for prion protein alleles encoding glutamine-171 renders sheep susceptible to natural scrapie."],"prefix":"10.1101","volume":"8","author":[{"given":"D","family":"Westaway","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Zuliani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C M","family":"Cooper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Da Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Neuman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A L","family":"Jenny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Detwiler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Prusiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1994,4,15]]},"reference":[{"key":"2021111418142099000_8.8.959.1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/154.3.518"},{"key":"2021111418142099000_8.8.959.2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90662-8"},{"key":"2021111418142099000_8.8.959.3","doi-asserted-by":"publisher","DOI":"10.1038\/321209a0"},{"key":"2021111418142099000_8.8.959.4","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.110.3.743"},{"key":"2021111418142099000_8.8.959.5","first-page":"6188","article-title":"Evidence for synthesis of scrapie prion proteins in the endocytic pathway.","volume":"267","year":"1992","journal-title":"J. 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