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The pyrosequencing-based method was evaluated by analyzing variation in copy number at the duplicated<jats:italic>KIT\/Dominant white<\/jats:italic>locus in pigs. We were able to distinguish haplotypes at this locus by combining the duplication breakpoint test with a diagnostic test for a functionally important splice mutation in the duplicated gene. An extensive allelic variation, including the presence of a new allele carrying a single<jats:italic>KIT<\/jats:italic>copy expected to encode a truncated KIT receptor, was revealed when analyzing white pigs from commercial lines.<\/jats:p>","DOI":"10.1101\/gr.1188203","type":"journal-article","created":{"date-parts":[[2003,9,10]],"date-time":"2003-09-10T17:35:19Z","timestamp":1063215319000},"page":"2171-2177","source":"Crossref","is-referenced-by-count":36,"title":["A Sensitive Method for Detecting Variation in Copy Numbers of Duplicated Genes"],"prefix":"10.1101","volume":"13","author":[{"given":"Gerli","family":"Pielberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy E.","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham S.","family":"Plastow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leif","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2003,9,2]]},"reference":[{"key":"2021111810531490000_13.9.2171.1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072047"},{"key":"2021111810531490000_13.9.2171.2","first-page":"158","volume":"148","year":"1990","journal-title":"Ciba Found. 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