{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:55:41Z","timestamp":1791158141600,"version":"4.1.0"},"reference-count":25,"publisher":"Cold Spring Harbor Laboratory","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genome Res."],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:p>SRY (sex-determining region of the Y chromosome) has recently been identified as a key regulatory gene in mammalian sex determination. The open reading frame of this gene contains an 80-amino-acid motif, the SRY-box, which shares a high degree of homology with a DNA-binding domain found in the high-mobility-group (HMG) proteins HMG1 and HMG2. The SRY box motif is highly conserved in several sequence-specific DNA-binding proteins that are known to act as transcription factors. Here we describe the use of degenerate PCR primers to identify SRY-related sequences containing the SRY-box motif from the genomic DNA of a variety of species. The results of this study suggest that in a diverse array of species SRY-related genes may serve as transcription factors that regulate a variety of developmental pathways, including sex determination.<\/jats:p>","DOI":"10.1101\/gr.2.3.218","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T21:18:07Z","timestamp":1181078287000},"page":"218-222","source":"Crossref","is-referenced-by-count":52,"title":["PCR amplification of SRY-related gene sequences reveals evolutionary conservation of the SRY-box motif."],"prefix":"10.1101","volume":"2","author":[{"given":"A M","family":"Coriat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J L","family":"Harry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Uwanogho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P T","family":"Sharpe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[1993,2,1]]},"reference":[{"key":"2021111810100369000_2.3.218.1","first-page":"150","article-title":"Expression of a candidate sex determining gene during mouse testis differentiation.","volume":"348","year":"1990","journal-title":"Nature"},{"key":"2021111810100369000_2.3.218.2","doi-asserted-by":"publisher","DOI":"10.1038\/348448A0"},{"key":"2021111810100369000_2.3.218.3","doi-asserted-by":"publisher","DOI":"10.1038\/348452a0"},{"key":"2021111810100369000_2.3.218.4","doi-asserted-by":"publisher","DOI":"10.1038\/351117a0"},{"key":"2021111810100369000_2.3.218.5","doi-asserted-by":"publisher","DOI":"10.1038\/346240a0"},{"key":"2021111810100369000_2.3.218.6","doi-asserted-by":"publisher","DOI":"10.1016\/0960-9822(92)90541-H"},{"key":"2021111810100369000_2.3.218.7","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1002\/j.1460-2075.1988.tb02973.x","article-title":"Four mating type genes control sexual differentiation in fission yeast.","volume":"7","year":"1988","journal-title":"EMBO J."},{"key":"2021111810100369000_2.3.218.8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.4917"},{"key":"2021111810100369000_2.3.218.9","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.11.1990"},{"key":"2021111810100369000_2.3.218.10","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.4.656"},{"key":"2021111810100369000_2.3.218.11","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.12b.2567"},{"key":"2021111810100369000_2.3.218.12","doi-asserted-by":"publisher","DOI":"10.1038\/344830a0"},{"key":"2021111810100369000_2.3.218.13","doi-asserted-by":"publisher","DOI":"10.1038\/354317a0"},{"key":"2021111810100369000_2.3.218.14","doi-asserted-by":"publisher","DOI":"10.1038\/346240a0"},{"key":"2021111810100369000_2.3.218.15","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1002\/j.1460-2075.1992.tb05144.x","article-title":"The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins.","volume":"11","year":"1992","journal-title":"EMBO J."},{"key":"2021111810100369000_2.3.218.16","doi-asserted-by":"publisher","DOI":"10.1126\/science.1734522"},{"key":"2021111810100369000_2.3.218.17","unstructured":"Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual 2nd ed., pp. 9.16\u00e2\u0080\u00939.19. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York."},{"key":"2021111810100369000_2.3.218.18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.3.1245"},{"key":"2021111810100369000_2.3.218.19","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1098\/rspb.1991.0060","article-title":"The isolation of conserved DNA sequences related to the human sex-determining region Y gene from the lesser black-backed gull (Larus fuscus).","volume":"244","year":"1991","journal-title":"Proc. R. Soc. Lond. 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Acta"}],"container-title":["Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/gr.2.3.218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T18:15:40Z","timestamp":1637259340000},"score":1,"resource":{"primary":{"URL":"http:\/\/genome.cshlp.org\/lookup\/doi\/10.1101\/gr.2.3.218"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,2]]}},"alternative-id":["10.1101\/gr.2.3.218"],"URL":"https:\/\/doi.org\/10.1101\/gr.2.3.218","relation":{},"ISSN":["1088-9051"],"issn-type":[{"value":"1088-9051","type":"print"}],"subject":[],"published":{"date-parts":[[1993,2]]}}}