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We used two-hybrid screening to map Smad signaling protein\u2013protein interactions and to establish a network of 755 interactions, involving 591 proteins, 179 of which were poorly or not annotated. The exploration of such complex interaction databases is improved by the use of PIMRider, a dedicated navigation tool accessible through the Web. The biological meaning of this network is illustrated by the presence of 18 known Smad-associated proteins. Functional assays performed in mammalian cells including siRNA knock-down experiments identified eight novel proteins involved in Smad signaling, thus validating this integrated functional proteomics approach.<\/jats:p>","DOI":"10.1101\/gr.2334104","type":"journal-article","created":{"date-parts":[[2004,7,9]],"date-time":"2004-07-09T22:20:25Z","timestamp":1089411625000},"page":"1324-1332","source":"Crossref","is-referenced-by-count":284,"title":["Functional Proteomics Mapping of a Human Signaling Pathway"],"prefix":"10.1101","volume":"14","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Colland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Jacq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Trouplin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Mougin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Groizeleau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Hamburger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Meil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Wojcik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Legrain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Gauthier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2004,7,1]]},"reference":[{"key":"2021111810542049000_14.7.1324.1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1996.0364"},{"key":"2021111810542049000_14.7.1324.2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"2021111810542049000_14.7.1324.3","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.1.37"},{"key":"2021111810542049000_14.7.1324.4","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"2021111810542049000_14.7.1324.5","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071809"},{"key":"2021111810542049000_14.7.1324.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(01)00344-3"},{"key":"2021111810542049000_14.7.1324.7","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.15.1880"},{"key":"2021111810542049000_14.7.1324.8","doi-asserted-by":"crossref","unstructured":"Bartel, P.L. 1993. 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