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Here, we investigate the effects of various vitamins (VB2, VB6, and VB12) on the mRNA m\n                    <jats:sup>6<\/jats:sup>\n                    A epitranscriptome in multiple mouse tissues (brain, liver, and testis). Clustering analyses reveal closer similarity between the brain and testis m\n                    <jats:sup>6<\/jats:sup>\n                    A profiles, whereas the liver exhibits a unique pattern, reflecting tissue-specific regulatory dynamics. More than 90% of m\n                    <jats:sup>6<\/jats:sup>\n                    A sites are independent of mRNA abundance, highlighting the post-transcriptional role of m\n                    <jats:sup>6<\/jats:sup>\n                    A modification. Additionally, alternative splicing (AS) variations reveal complex interactions between vitamins, m\n                    <jats:sup>6<\/jats:sup>\n                    A modification, and AS, with tissue- and vitamin-specific effects on biological pathways. We identify 22 comethylation modules, associated with pathways such as neurodegenerative diseases and immune regulation. Key vitamin-responsive genes are found as central regulators of m\n                    <jats:sup>6<\/jats:sup>\n                    A dynamics, aligning with known roles of vitamins in metabolism, neural plasticity, and gene expression. Together, our findings provide the first comprehensive atlas of vitamin-driven, tissue-specific m\n                    <jats:sup>6<\/jats:sup>\n                    A modifications, offering new insights into the post-transcriptional regulatory mechanisms underlying vitamin-mediated cellular functions and their implications for nutritional or pharmacological modulation of the epitranscriptome in health and disease.\n                  <\/jats:p>","DOI":"10.1101\/gr.280968.125","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T20:15:13Z","timestamp":1778530513000},"page":"1141-1157","source":"Crossref","is-referenced-by-count":0,"title":["Tissue-specific mRNA m\n                    <sup>6<\/sup>\n                    A reprogramming unveils vitamin-driven post-transcriptional regulation in 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Sciences, South China Normal University, Guangzhou 510631, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life Sciences, South China Normal University, Guangzhou 510631, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqi","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Life Sciences, South China Normal University, Guangzhou 510631, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Tian","sequence":"additional","affiliation":[{"name":"Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjun","family":"Wen","sequence":"additional","affiliation":[{"name":"Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, 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