{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:47:47Z","timestamp":1785782867442,"version":"3.56.0"},"reference-count":46,"publisher":"Cold Spring Harbor Laboratory","issue":"8","funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"grant-in-aid for scientific research [A]","award":["JP22H00413"],"award-info":[{"award-number":["JP22H00413"]}]},{"name":"grant-in-aid for scientific research on innovative areas","award":["JP18H05530"],"award-info":[{"award-number":["JP18H05530"]}]},{"name":"grant-in-aid for early-career scientists","award":["22K15044"],"award-info":[{"award-number":["22K15044"]}]},{"name":"grant in aid for challenging research [pioneering]","award":["24K21266"],"award-info":[{"award-number":["24K21266"]}]},{"name":"grant-in-aid for transformative research areas [A]","award":["23H04956"],"award-info":[{"award-number":["23H04956"]}]},{"DOI":"10.13039\/501100003382","name":"The Japan Science and Technology Agency","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003382","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Fusion Oriented REsearch for disruptive Science and Technology [FOREST] program","award":["JPMJFR221G"],"award-info":[{"award-number":["JPMJFR221G"]}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genome Res."],"accepted":{"date-parts":[[2026,6,16]]},"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Retroelement silencing factor 1 (\n                    <jats:italic>Resf1<\/jats:italic>\n                    ) is involved in retroelement silencing in cooperation with H3K9 methyltransferase SETDB1 by regulating H3K9 methylation in mouse embryonic stem cells (mESCs). However, it remains unknown whether\n                    <jats:italic>Resf1<\/jats:italic>\n                    functions in retroelement silencing in vivo and has a role in development. Here, we established\n                    <jats:italic>Resf1<\/jats:italic>\n                    -deficient mice, which exhibit developmental delay, partial embryonic lethality, and placental defects. Notably, retroelements are also upregulated in the\n                    <jats:italic>Resf1<\/jats:italic>\n                    -deficient placenta, correlating with increased expression of nearby genes. To further assess whether\n                    <jats:italic>Resf1<\/jats:italic>\n                    functions within the trophoblast lineage, we generate\n                    <jats:italic>Resf1<\/jats:italic>\n                    -deficient trophoblast stem cell (TSC) lines. Both undifferentiated TSCs and differentiated TSCs (D-TSCs) display increased retroelement expression along with elevated levels of genes associated with placental development. Moreover,\n                    <jats:italic>Resf1<\/jats:italic>\n                    -deficient TSCs exhibit compromised maintenance of H3K9me3 domains in a manner independent of SETDB1. Collectively, our findings reveal that\n                    <jats:italic>Resf1<\/jats:italic>\n                    plays multifaceted roles beyond retroelement silencing, underscoring its importance in development and its critical function in trophoblast lineage regulation.\n                  <\/jats:p>","DOI":"10.1101\/gr.281507.125","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T15:45:12Z","timestamp":1782747912000},"page":"1548-1560","source":"Crossref","is-referenced-by-count":0,"title":["<i>Resf1<\/i>\n                    is required for proper placental development and configuration of trophoblast cell\u2013specific heterochromatin"],"prefix":"10.1101","volume":"36","author":[{"given":"Kei","family":"Fukuda","sequence":"first","affiliation":[{"name":"Cellular Memory Laboratory, RIKEN Pioneering Research Institute, Wako 351-0198, Japan;"},{"name":"Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, 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