{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:39:18Z","timestamp":1771663158809,"version":"3.50.1"},"reference-count":66,"publisher":"Cold Spring Harbor Laboratory","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genome Res."],"published-print":{"date-parts":[[2002,5,1]]},"abstract":"<jats:p>The stem cell leukemia (<jats:italic>SCL<\/jats:italic>) gene encodes a bHLH transcription factor with a pivotal role in hematopoiesis and vasculogenesis and a pattern of expression that is highly conserved between mammals and zebrafish. Here we report the isolation and characterization of the zebrafish <jats:italic>SCL<\/jats:italic> locus together with the identification of three neighboring genes, <jats:italic>IER5<\/jats:italic>,<jats:italic>MAP17<\/jats:italic>, and <jats:italic>MUPP1<\/jats:italic>. This region spans 68 kb and comprises the longest zebrafish genomic sequence currently available for comparison with mammalian, chicken, and pufferfish sequences. Our data show conserved synteny between zebrafish and mammalian <jats:italic>SCL<\/jats:italic> and <jats:italic>MAP17<\/jats:italic> loci, thus suggesting the likely genomic domain necessary for the conserved pattern of<jats:italic>SCL<\/jats:italic> expression. Long-range comparative sequence analysis\/phylogenetic footprinting was used to identify noncoding conserved sequences representing candidate transcriptional regulatory elements. The <jats:italic>SCL<\/jats:italic> promoter\/enhancer, exon 1, and the poly(A) region were highly conserved, but no homology to other known mouse<jats:italic>SCL<\/jats:italic> enhancers was detected in the zebrafish sequence. A combined homology\/structure analysis of the poly(A) region predicted consistent structural features, suggesting a conserved functional role in mRNA regulation. Analysis of the <jats:italic>SCL<\/jats:italic> promoter\/enhancer revealed five motifs, which were conserved from zebrafish to mammals, and each of which is essential for the appropriate pattern or level of<jats:italic>SCL<\/jats:italic> transcription.<\/jats:p><jats:p>[The following individuals kindly provided reagents, samples, or unpublished information as indicated in the paper: N. Tanese.]<\/jats:p>","DOI":"10.1101\/gr.45502","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T00:00:38Z","timestamp":1027728038000},"page":"749-759","source":"Crossref","is-referenced-by-count":72,"title":["Transcriptional Regulation of the Stem Cell Leukemia Gene (<i>SCL<\/i>) \u2014 Comparative Analysis of Five Vertebrate SCL Loci"],"prefix":"10.1101","volume":"12","author":[{"given":"Berthold","family":"G\u00f6ttgens","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda M.","family":"Barton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Chapman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angus M.","family":"Sinclair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjarne","family":"Knudsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren","family":"Grafham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James G.R.","family":"Gilbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Rogers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David R.","family":"Bentley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony R.","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2002,5,1]]},"reference":[{"key":"2021111810465559000_12.5.749.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00166-5"},{"key":"2021111810465559000_12.5.749.2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"2021111810465559000_12.5.749.3","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9462"},{"key":"2021111810465559000_12.5.749.4","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.6.1982-1992.2000"},{"key":"2021111810465559000_12.5.749.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1684"},{"key":"2021111810465559000_12.5.749.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.101532998"},{"key":"2021111810465559000_12.5.749.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(99)00210-7"},{"key":"2021111810465559000_12.5.749.8","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.0327"},{"key":"2021111810465559000_12.5.749.9","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1182\/blood.V93.9.2760","article-title":"The SCL gene: From case report to critical hematopoietic regulator.","volume":"93","author":"Begley","year":"1999","journal-title":"Blood"},{"key":"2021111810465559000_12.5.749.10","doi-asserted-by":"crossref","first-page":"1502","DOI":"10.1182\/blood.V86.4.1502.bloodjournal8641502","article-title":"Lineage-restricted regulation of the murine SCL\/TAL-1 promoter.","volume":"86","author":"Bockamp","year":"1995","journal-title":"Blood"},{"key":"2021111810465559000_12.5.749.11","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.13.8781"},{"key":"2021111810465559000_12.5.749.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.44.29032"},{"key":"2021111810465559000_12.5.749.13","doi-asserted-by":"crossref","first-page":"1671","DOI":"10.1182\/blood.V95.5.1671.005k39_1671_1679","article-title":"Vasculogenesis in the day 6.5 to 9.5 mouse embryo.","volume":"95","author":"Drake","year":"2000","journal-title":"Blood"},{"key":"2021111810465559000_12.5.749.14","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(94)90091-4"},{"key":"2021111810465559000_12.5.749.15","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.10.2315"},{"key":"2021111810465559000_12.5.749.16","doi-asserted-by":"publisher","DOI":"10.1038\/83807"},{"key":"2021111810465559000_12.5.749.17","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)61405-3"},{"key":"2021111810465559000_12.5.749.18","doi-asserted-by":"crossref","first-page":"3551","DOI":"10.1002\/j.1460-2075.1994.tb06662.x","article-title":"The degree of variation in DNA sequence recognition among four Drosophila homeotic proteins.","volume":"13","author":"Ekker","year":"1994","journal-title":"EMBO J."},{"key":"2021111810465559000_12.5.749.19","doi-asserted-by":"crossref","first-page":"3754","DOI":"10.1182\/blood.V94.11.3754","article-title":"SCL expression in the mouse embryo detected with a targeted lacZ reporter gene demonstrates its localization to hematopoietic, vascular, and neural tissues.","volume":"94","author":"Elefanty","year":"1999","journal-title":"Blood"},{"key":"2021111810465559000_12.5.749.20","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.4.371"},{"key":"2021111810465559000_12.5.749.21","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1101\/gr.9.3.251","article-title":"Analysis of 148 kb of genomic DNA around the wnt1 locus of Fugu rubripes.","volume":"9","author":"Gellner","year":"1999","journal-title":"Genome Res."},{"key":"2021111810465559000_12.5.749.22","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.14.4029"},{"key":"2021111810465559000_12.5.749.23","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201426"},{"key":"2021111810465559000_12.5.749.24","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.5162"},{"key":"2021111810465559000_12.5.749.25","doi-asserted-by":"publisher","DOI":"10.1038\/72635"},{"key":"2021111810465559000_12.5.749.26","doi-asserted-by":"publisher","DOI":"10.1101\/gr.153001"},{"key":"2021111810465559000_12.5.749.27","first-page":"653","article-title":"SCL is co-expressed with GATA-1 in haemopoietic cells but is also expressed in developing brain.","volume":"7","author":"Green","year":"1992","journal-title":"Oncogene"},{"key":"2021111810465559000_12.5.749.28","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90620-R"},{"key":"2021111810465559000_12.5.749.29","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.21.4457"},{"key":"2021111810465559000_12.5.749.30","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2801"},{"key":"2021111810465559000_12.5.749.31","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1182\/blood.V83.5.1200.1200","article-title":"The SCL\/TAL-1 gene is expressed in progenitors of both the hematopoietic and vascular systems during embryogenesis.","volume":"83","author":"Kallianpur","year":"1994","journal-title":"Blood"},{"key":"2021111810465559000_12.5.749.32","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/15.6.446"},{"key":"2021111810465559000_12.5.749.33","first-page":"493","article-title":"Identification and partial characterization of a novel membrane-associated protein (MAP17) up-regulated in human carcinomas and modulating cell replication and tumor growth.","volume":"149","author":"Kocher","year":"1996","journal-title":"Am. J. Pathol."},{"key":"2021111810465559000_12.5.749.34","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90290-9"},{"key":"2021111810465559000_12.5.749.35","first-page":"2623","article-title":"GATA- and SP1-binding sites are required for the full activity of the tissue-specific promoter of the tal-1 gene.","volume":"9","author":"Lecointe","year":"1994","journal-title":"Oncogene"},{"key":"2021111810465559000_12.5.749.36","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.7.1019"},{"key":"2021111810465559000_12.5.749.37","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.5.621"},{"key":"2021111810465559000_12.5.749.38","doi-asserted-by":"publisher","DOI":"10.1002\/bies.950171007"},{"key":"2021111810465559000_12.5.749.39","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/16.11.1046"},{"key":"2021111810465559000_12.5.749.40","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1242\/dev.125.14.2611","article-title":"SCL specifies hematopoietic mesoderm in Xenopus embryos.","volume":"125","author":"Mead","year":"1998","journal-title":"Development"},{"key":"2021111810465559000_12.5.749.41","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.3.290"},{"key":"2021111810465559000_12.5.749.42","first-page":"131","article-title":"Discordant regulation of SCL\/TAL-1 mRNA and protein during erythroid differentiation.","volume":"11","author":"Murrell","year":"1995","journal-title":"Oncogene"},{"key":"2021111810465559000_12.5.749.43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.9.4712"},{"key":"2021111810465559000_12.5.749.44","first-page":"1784s","article-title":"Intersections between blood cell development and leukemia genes.","volume":"59","author":"Orkin","year":"1999","journal-title":"Cancer Res."},{"key":"2021111810465559000_12.5.749.45","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.12.3461"},{"key":"2021111810465559000_12.5.749.46","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(05)80008-X"},{"key":"2021111810465559000_12.5.749.47","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80076-8"},{"key":"2021111810465559000_12.5.749.48","doi-asserted-by":"publisher","DOI":"10.1101\/gr.164800"},{"key":"2021111810465559000_12.5.749.49","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.19.11603"},{"key":"2021111810465559000_12.5.749.50","doi-asserted-by":"crossref","first-page":"4123","DOI":"10.1002\/j.1460-2075.1996.tb00787.x","article-title":"The SCL gene product is required for the generation of all hematopoietic lineages in the adult mouse.","volume":"15","author":"Robb","year":"1996","journal-title":"EMBO J."},{"key":"2021111810465559000_12.5.749.51","doi-asserted-by":"crossref","first-page":"2447","DOI":"10.1242\/dev.127.11.2447","article-title":"A transitional stage in the commitment of mesoderm to hematopoiesis requiring the transcription factor SCL\/tal-1.","volume":"127","author":"Robertson","year":"2000","journal-title":"Development"},{"key":"2021111810465559000_12.5.749.52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.131199098"},{"key":"2021111810465559000_12.5.749.53","doi-asserted-by":"crossref","first-page":"2735","DOI":"10.1242\/dev.125.14.2735","article-title":"Identification of an evolutionary conserved 110 base-pair cis-acting regulatory sequence that governs wnt-1 expressino in the murine neural plate.","volume":"125","author":"Rowitch","year":"1998","journal-title":"Development"},{"key":"2021111810465559000_12.5.749.54","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1242\/dev.126.17.3891","article-title":"An SCL 3\u2032 enhancer targets developing endothelium together with embryonic and adult hematopoietic progenitors.","volume":"126","author":"Sanchez","year":"1999","journal-title":"Development"},{"key":"2021111810465559000_12.5.749.55","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.12.2141"},{"key":"2021111810465559000_12.5.749.56","doi-asserted-by":"publisher","DOI":"10.1101\/gr.10.4.577"},{"key":"2021111810465559000_12.5.749.57","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9236"},{"key":"2021111810465559000_12.5.749.58","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(95)00714-8"},{"key":"2021111810465559000_12.5.749.59","doi-asserted-by":"crossref","first-page":"7371","DOI":"10.1128\/MCB.18.12.7371","article-title":"Highly conserved RNA sequences that are sensors of environmental stress.","volume":"18","author":"Spicher","year":"1998","journal-title":"Mol. Cell. Biol."},{"key":"2021111810465559000_12.5.749.60","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00261-9"},{"key":"2021111810465559000_12.5.749.61","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00141-0"},{"key":"2021111810465559000_12.5.749.62","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.4.473"},{"key":"2021111810465559000_12.5.749.63","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5679"},{"key":"2021111810465559000_12.5.749.64","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5358.1896"},{"key":"2021111810465559000_12.5.749.65","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1523\/JNEUROSCI.20-02-00709.2000","article-title":"A highly conserved enhancer in the Dlx5\/Dlx6 intergenic region is the site of cross-regulatory interactions between Dlx genes in the embryonic forebrain.","volume":"20","author":"Zerucha","year":"2000","journal-title":"J. Neurosci."},{"key":"2021111810465559000_12.5.749.66","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1128\/MCB.16.3.1231","article-title":"CCAAT enhancer-binding protein (C\/EBP) and AML1 (CBF alpha2) synergistically activate the macrophage colony-stimulating factor receptor promoter.","volume":"16","author":"Zhang","year":"1996","journal-title":"Mol. Cell. Biol."}],"container-title":["Genome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/gr.45502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,18]],"date-time":"2021-11-18T19:12:28Z","timestamp":1637262748000},"score":1,"resource":{"primary":{"URL":"http:\/\/genome.cshlp.org\/lookup\/doi\/10.1101\/gr.45502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5,1]]},"references-count":66,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,5,1]]}},"alternative-id":["10.1101\/gr.45502"],"URL":"https:\/\/doi.org\/10.1101\/gr.45502","relation":{},"ISSN":["1088-9051","1549-5469"],"issn-type":[{"value":"1088-9051","type":"print"},{"value":"1549-5469","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,5,1]]}}}