{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T23:15:49Z","timestamp":1700262949214},"reference-count":27,"publisher":"Cold Spring Harbor Laboratory","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genome Res."],"published-print":{"date-parts":[[1998,7,1]]},"abstract":"<jats:p>Palmitoyl protein thioesterase (PPT) is the defective enzyme in infantile neuronal ceroid lipofuscinosis (INCL), which is a recessively inherited, progressive neurodegenerative disorder. We present here the cloning, chromosomal mapping, genomic structure, and the expression of the cDNA of mouse <jats:italic>PPT.<\/jats:italic> The mouse <jats:italic>PPT<\/jats:italic> gene spans &gt;21 kb of genomic DNA and contains nine exons with a coding sequence of 918 bp. Fluorescence in situ hybridization to metaphase chromosomes localized the mouse <jats:italic>PPT<\/jats:italic> gene to the chromosome 4 conserved syntenic region with human chromosome 1p32 where the human <jats:italic>PPT<\/jats:italic>is located. <jats:italic>PPT<\/jats:italic> is expressed widely in a variety of mouse tissues. The mouse <jats:italic>PPT<\/jats:italic> cDNA is conserved highly with the human and rat <jats:italic>PPT<\/jats:italic> both at the nucleotide and amino acid sequence level. Transient expression of mouse <jats:italic>PPT<\/jats:italic> in COS-1 cells yielded a 38\/36-kD differentially glycosylated polypeptide that was also secreted into culture media. Immunofluorescence analysis of transiently transfected HeLa cells indicated lysosomal localization of mouse <jats:italic>PPT.<\/jats:italic> Based on the high conservation of the gene and polypeptide structure as well as similar processing and intracellular localization, the function of <jats:italic>PPT<\/jats:italic> in mouse and human are likely to be very similar.<\/jats:p><jats:p>[The sequence data described in this paper have been submitted to GenBank under accession no. AF071O25.]<\/jats:p>","DOI":"10.1101\/gr.8.7.724","type":"journal-article","created":{"date-parts":[[2017,5,3]],"date-time":"2017-05-03T00:31:56Z","timestamp":1493771516000},"page":"724-730","source":"Crossref","is-referenced-by-count":11,"title":["Mouse Palmitoyl Protein Thioesterase: Gene Structure and Expression of\u2009cDNA"],"prefix":"10.1101","volume":"8","author":[{"given":"Tarja","family":"Salonen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elina","family":"Hellsten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Horelli-Kuitunen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leena","family":"Peltonen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anu","family":"Jalanko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"2021111810280628000_8.7.724.1","doi-asserted-by":"crossref","first-page":"8222","DOI":"10.1016\/S0021-9258(18)83172-6","article-title":"Cloning, structure and expression of the mitochondrial cytochrome P-450 sterol 26-hydroxylase, a bile acid biosynthetic enzyme.","volume":"264","author":"Anderson","year":"1989","journal-title":"J. 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