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A large number of transcriptional units exist within the defined critical region, many of which have been identified previously by direct selection. However, no single obvious candidate gene for the VCFS\/DGS phenotype has yet been found. Our technique has been applied to the DiGeorge critical region and has resulted in the isolation of a novel candidate gene, <jats:italic>Cdc45l2,<\/jats:italic> similar to yeast Cdc45p.<\/jats:p><jats:p>[The sequence data described in this paper have been submitted to the EMBL data library under accession nos. 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