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Here, using a light\/dark preference paradigm with well-controlled luminance history and constant visual surrounding in larval zebrafish, we analyzed intra- and intertrial dynamics for two behavioral components, dark avoidance and center avoidance. We uncover that larval zebrafish display short-term learning of dark avoidance with initial sensitization followed by habituation; they also exhibit long-term learning that is sensitive to trial interval length. We further show that such stereotyped learning patterns is stimulus-specific, as they are not observed for center avoidance. Finally, we demonstrate at individual levels that long-term learning is under homeostatic control. Together, our work has established a novel paradigm to understand learning, uncovered sequential sensitization and habituation, and demonstrated stimulus specificity, individuality, as well as dynamicity in learning.<\/jats:p>","DOI":"10.1101\/lm.053425.121","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T11:00:16Z","timestamp":1623754816000},"page":"228-238","source":"Crossref","is-referenced-by-count":6,"title":["Larval zebrafish display dynamic learning of aversive stimuli in a constant visual surrounding"],"prefix":"10.1101","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7602-5824","authenticated-orcid":false,"given":"Jiale","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romelo","family":"Casanave","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2021,6,15]]},"reference":[{"key":"2021111208144854000_28.7.228.1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2434"},{"key":"2021111208144854000_28.7.228.2","doi-asserted-by":"publisher","DOI":"10.3791\/52615"},{"key":"2021111208144854000_28.7.228.3","doi-asserted-by":"publisher","DOI":"10.1101\/lm.041830.116"},{"key":"2021111208144854000_28.7.228.4","doi-asserted-by":"publisher","DOI":"10.1186\/s12868-016-0298-z"},{"key":"2021111208144854000_28.7.228.5","doi-asserted-by":"publisher","DOI":"10.1038\/sj.npp.1301489"},{"key":"2021111208144854000_28.7.228.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpsycho.2014.01.001"},{"key":"2021111208144854000_28.7.228.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2016.05.012"},{"key":"2021111208144854000_28.7.228.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.nlm.2009.02.002"},{"key":"2021111208144854000_28.7.228.9","doi-asserted-by":"publisher","DOI":"10.1007\/BF00309213"},{"key":"2021111208144854000_28.7.228.10","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(03)01274-3"},{"key":"2021111208144854000_28.7.228.11","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.003939"},{"key":"2021111208144854000_28.7.228.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.01.022"},{"key":"2021111208144854000_28.7.228.13","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a021675"},{"key":"2021111208144854000_28.7.228.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2012.07.038"},{"key":"2021111208144854000_28.7.228.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.01.007"},{"key":"2021111208144854000_28.7.228.16","doi-asserted-by":"publisher","DOI":"10.1186\/1129-2377-14-65"},{"key":"2021111208144854000_28.7.228.17","doi-asserted-by":"publisher","DOI":"10.1080\/01677063.2019.1663846"},{"key":"2021111208144854000_28.7.228.18","doi-asserted-by":"publisher","DOI":"10.1037\/h0032811"},{"key":"2021111208144854000_28.7.228.19","unstructured":"Everitt B , Skrondal A . 2002. 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