{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T05:30:05Z","timestamp":1770528605958,"version":"3.49.0"},"reference-count":39,"publisher":"Cold Spring Harbor Laboratory","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Mol Case Stud"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:p>Schwannomatosis is a rare genetic disorder that predisposes individuals to development of multiple schwannomas mainly in spinal and peripheral nerves and to debilitating chronic pain often unrelated to any schwannoma. Pathogenic variants of two genes, <jats:italic>SMARCB1<\/jats:italic> and <jats:italic>LZTR1<\/jats:italic>, are causal in familial cases. However, many schwannomatosis patients lack mutations in these genes. Surgery is the standard treatment for schwannomas but leaves patients with increasing neurological deficits. Pain management is a daily struggle controlled by the use of multiple analgesic and anti-inflammatory drugs. There is a need for both nonsurgical treatment to manage tumor growth and nonaddictive, nonsedative pain control. Because standard clinical trials are exceedingly difficult for patients with rare disorders, precision medicine approaches offer the possibility of bespoke therapeutic regimens to control tumor growth. As a proof of principle, we obtained a bio-specimen of paraspinal schwannoma from a schwannomatosis patient with a germline point mutation in the <jats:italic>SMARCB1\/INI<\/jats:italic> gene. We created an <jats:italic>hTERT<\/jats:italic> immortalized cell line and tested the ability of targeted small molecules with efficacy in neurofibromatosis type 2\u2013related schwannomas to reduce cell viability and induce cell death. We identified WP1066, a STAT3 inhibitor, currently in phase 2 clinical trials for pediatric and adult brain tumors as a lead compound. It reduced cell viability and STAT-3 phosphorylation and induced expression of markers for both necroptosis and caspase-dependent cell death. The results demonstrate feasibility in creating patient-derived cell lines for use in precision medicine studies.<\/jats:p>","DOI":"10.1101\/mcs.a006178","type":"journal-article","created":{"date-parts":[[2022,6,22]],"date-time":"2022-06-22T19:05:15Z","timestamp":1655924715000},"page":"a006178","source":"Crossref","is-referenced-by-count":6,"title":["WP1066 induces cell death in a schwannomatosis patient\u2013derived schwannoma cell 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