{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,4]],"date-time":"2024-08-04T20:33:21Z","timestamp":1722803601259},"reference-count":39,"publisher":"Cold Spring Harbor Laboratory","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>INTRODUCTION<\/jats:title><jats:p><jats:italic>Pristionchus pacificus<\/jats:italic> is a nematode that has been established as a model system for evolutionary developmental biology. Initially, <jats:italic>P. pacificus<\/jats:italic> was used as a convenient nematode with which to compare the processes of vulva and gonad development as well as sex determination to <jats:italic>Caenorhabditis elegans<\/jats:italic>, one of the best-studied animal models. <jats:italic>P. pacificus<\/jats:italic> shares many features with <jats:italic>C. elegans<\/jats:italic>, including a short generation time, its ability to be easily cultured in the laboratory, and self-fertilization as a mode of reproduction. These features allowed forward and reverse genetic tools to be developed for this species. The application of these tools for genetic and molecular analysis of vulva formation revealed substantial differences between <jats:italic>P. pacificus<\/jats:italic> and <jats:italic>C. elegans<\/jats:italic>. The genome of <jats:italic>P. pacificus<\/jats:italic> has recently been sequenced and showed an expansion of protein-coding genes compared with <jats:italic>C. elegans<\/jats:italic>. Interestingly, the <jats:italic>P. pacificus<\/jats:italic> genome encodes some genes, such as cellulases, that are known to be present only in plant-parasitic nematodes. Many of the putative functions of the predicted genes in the genome are related to the ecology of <jats:italic>P. pacificus<\/jats:italic> and other <jats:italic>Pristionchus<\/jats:italic> species. <jats:italic>Pristionchus<\/jats:italic> nematodes can be isolated from beetles and soil, indicating that the ecology of <jats:italic>P. pacificus<\/jats:italic> is strikingly different from that of <jats:italic>C. elegans<\/jats:italic>. Generally, <jats:italic>Pristionchus<\/jats:italic> species show an unexpected level of species specificity in their beetle associations, providing a unique opportunity to study the genetic and molecular mechanisms underlying the interactions of organisms in the environment. Thus, <jats:italic>P. pacificus<\/jats:italic> is not only an established model system for evolutionary developmental biology, but also an emerging model system for the evolution of complex life-history traits.<\/jats:p>","DOI":"10.1101\/pdb.emo102","type":"journal-article","created":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T17:29:59Z","timestamp":1222882199000},"page":"pdb.emo102","source":"Crossref","is-referenced-by-count":4,"title":["<i>Pristionchus pacificus<\/i>: A Genetic Model System for the Study of Evolutionary Developmental Biology and the Evolution of Complex Life-History Traits"],"prefix":"10.1101","volume":"2008","author":[{"given":"Robbie","family":"Rae","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin","family":"Schlager","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf J.","family":"Sommer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2010,12,3]]},"reference":[{"key":"2021111008043418000_2008.10.pdb.emo102.1","doi-asserted-by":"publisher","DOI":"10.1038\/ng"},{"key":"2021111008043418000_2008.10.pdb.emo102.2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5337.452"},{"key":"2021111008043418000_2008.10.pdb.emo102.3","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01531-3"},{"key":"2021111008043418000_2008.10.pdb.emo102.4","first-page":"95","article-title":"Pristionchus uniformis sp. 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