{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:47:54Z","timestamp":1764402474523},"reference-count":53,"publisher":"Cold Spring Harbor Laboratory","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>INTRODUCTION<\/jats:title><jats:p>Blood-feeding mosquitoes, including the dengue and yellow fever vector<jats:italic>Aedes aegypti<\/jats:italic>, transmit many of the world\u2019s deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects. Targets of particular interest include genes that regulate development. However, although the<jats:italic>Ae. aegypti<\/jats:italic>genome project uncovered homologs of many known developmental regulatory genes, little is known of the genetic regulation of development in<jats:italic>Ae. aegypti<\/jats:italic>or other vector mosquitoes. This article provides an overview of the background, husbandry, and potential uses of<jats:italic>Ae. aegypti<\/jats:italic>as a model species. Methods for culturing, collecting and fixing developing tissues, analyzing gene and protein expression, and knocking down genes are permitting detailed analyses of the functions of developmental regulatory genes and the selective inhibition of such genes during<jats:italic>Ae. aegypti<\/jats:italic>development. This methodology, much of which is applicable to other mosquito species, is useful to both the comparative development and vector research communities.<\/jats:p>","DOI":"10.1101\/pdb.emo141","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T14:23:09Z","timestamp":1285942989000},"page":"pdb.emo141","source":"Crossref","is-referenced-by-count":40,"title":["<i>Aedes aegypti<\/i>: An Emerging Model for Vector Mosquito Development: Figure 1."],"prefix":"10.1101","volume":"2010","author":[{"given":"Anthony","family":"Clemons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgan","family":"Haugen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Flannery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Tomchaney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristopher","family":"Kast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin","family":"Jacowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christy","family":"Le","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Mori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Simanton Holland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Sarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Severson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly","family":"Duman-Scheel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2010,10,1]]},"reference":[{"key":"2021111008193379000_2010.10.pdb.emo141.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00028-2"},{"key":"2021111008193379000_2010.10.pdb.emo141.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0701515104"},{"key":"2021111008193379000_2010.10.pdb.emo141.3","doi-asserted-by":"crossref","first-page":"1727","DOI":"10.1093\/genetics\/143.4.1727","article-title":"Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers","volume":"143","author":"Antolin","year":"1996","journal-title":"Genetics"},{"key":"2021111008193379000_2010.10.pdb.emo141.4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2005.00560.x"},{"key":"2021111008193379000_2010.10.pdb.emo141.5","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1093\/genetics\/156.2.687","article-title":"Quantitative trait loci that control vector competence for dengue-2 virus in the mosquito Aedes aegypti","volume":"156","author":"Bosio","year":"2000","journal-title":"Genetics"},{"key":"2021111008193379000_2010.10.pdb.emo141.6","doi-asserted-by":"crossref","unstructured":"Brown AE Catteruccia F (2006) Toward silencing the burden of malaria: Progress and prospects for RNAi-based approaches. 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