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Also discussed are special breeding techniques to delete a selection cassette in vivo, if you produced the mutation in embryonic stem (ES) cells, and to maintain a mutant with a balancer chromosome. In the event of either male or female infertility in the heterozygotes, assisted reproductive techniques may be necessary.<\/jats:p>","DOI":"10.1101\/pdb.over107960","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T06:10:11Z","timestamp":1706508611000},"page":"pdb.over107960","source":"Crossref","is-referenced-by-count":1,"title":["Strategies for Maintaining Mouse Mutations"],"prefix":"10.1101","volume":"2024","author":[{"given":"Virginia E.","family":"Papaioannou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard R.","family":"Behringer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"key":"2024012822100686000_2024.1.pdb.over107960.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81527-5"},{"key":"2024012822100686000_2024.1.pdb.over107960.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot094458"},{"key":"2024012822100686000_2024.1.pdb.over107960.3","unstructured":"Behringer RR , Gertsenstein M , Nagy KV , Nagy A . 2014. 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