{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,2,21]],"date-time":"2023-02-21T01:28:27Z","timestamp":1676942907751},"reference-count":11,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:p>Intrinsic optical changes (light scattering signals) occur in mammalian nerve terminals during and immediately following the arrival of the action potential. In the neurohypophysis (posterior pituitary gland), the action potential is coupled to calcium-mediated secretion of the neuropeptides oxytocin and vasopressin. This excitation\u2013secretion coupling is intimately related to extremely rapid changes in light scattering. These optical signals provide a millisecond\u2013time-resolved monitor of events in the terminals that follow the arrival of the action potential and the entry of calcium. Light scattering procedures are designed to measure intrinsic optical signals from mammalian nerve terminals. In practice, these signals are remarkably simple to record from any of the mammalian neurohypophyses that have been studied. To date, this approach has been used successfully in mouse, rat, and guinea pig. This protocol provides instrumentation requirements and a method for preparation of the neurohypophysis so that intrinsic optical signals can be measured from nerve terminals. It also includes a discussion of the interpretation of the signals that are obtained.<\/jats:p>","DOI":"10.1101\/pdb.prot072355","type":"journal-article","created":{"date-parts":[[2012,12,3]],"date-time":"2012-12-03T16:03:36Z","timestamp":1354550616000},"page":"pdb.prot072355","source":"Crossref","is-referenced-by-count":1,"title":["Measuring Intrinsic Optical Signals from Mammalian Nerve Terminals"],"prefix":"10.1101","volume":"2012","author":[{"given":"Brian M.","family":"Salzberg","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Muschol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Kosterin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Lia","family":"Obaid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2012,12,3]]},"reference":[{"key":"2021111008394517000_2012.12.pdb.prot072355.1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1113\/jphysiol.1972.sp009919","article-title":"Changes in light scattering that accompany the action potential in squid giant axons: Potential-dependent components","volume":"224","year":"1972","journal-title":"J Physiol"},{"key":"2021111008394517000_2012.12.pdb.prot072355.2","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1113\/jphysiol.1972.sp009920","article-title":"Changes in light scattering that accompany the action potential in squid giant axons: Current-dependent components","volume":"224","year":"1972","journal-title":"J Physiol"},{"key":"2021111008394517000_2012.12.pdb.prot072355.3","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1159\/000124582","article-title":"Action potentials and frequency-dependent secretion in the mouse neurohypophysis","volume":"43","year":"1986","journal-title":"Neuroendocrinology"},{"key":"2021111008394517000_2012.12.pdb.prot072355.4","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.103754"},{"key":"2021111008394517000_2012.12.pdb.prot072355.5","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.107.3.353"},{"key":"2021111008394517000_2012.12.pdb.prot072355.6","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.93.4.715"},{"key":"2021111008394517000_2012.12.pdb.prot072355.7","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.99.4.491"},{"key":"2021111008394517000_2012.12.pdb.prot072355.8","first-page":"771","article-title":"Electrophysiology of hypothalamic magnocellular neurons secreting oxytocin and vasopressin","volume":"7","year":"1982","journal-title":"Neuroscience"},{"key":"2021111008394517000_2012.12.pdb.prot072355.9","first-page":"A227","article-title":"Triggered calcium release from intraterminal stores may play a direct role in neuropeptide secretion: Evidence from light scattering in mammalian nerve terminals","volume":"72","year":"1997","journal-title":"Biophys J"},{"key":"2021111008394517000_2012.12.pdb.prot072355.10","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1152\/jn.1977.40.6.1281","article-title":"Optical recording of neuronal activity in an invertebrate central nervous system: Simultaneous monitoring of several neurons","volume":"40","year":"1977","journal-title":"J Neurophysiol"},{"key":"2021111008394517000_2012.12.pdb.prot072355.11","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.86.3.395"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot072355","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T18:53:44Z","timestamp":1636570424000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot072355"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12]]},"references-count":11,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2012,12,3]]},"published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1101\/pdb.prot072355"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot072355","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12]]}}}