{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:40:00Z","timestamp":1791330000633,"version":"4.3.1"},"reference-count":1,"publisher":"Cold Spring Harbor Laboratory","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Immunopanning of viable neurons requires availability of an antibody directed against a neuronal surface epitope that is specific for a neuron type of interest (e.g., corticospinal motor neurons [CSMNs]) and that is able to immobilize only neurons of interest from a dissociated brain suspension on a culture dish. However, few, if any, neuron type\u2013specific monoclonal antibodies have been developed. Panning antibodies have not been characterized for CSMNs. To circumvent this issue, we developed a method to retrograde label CSMNs with a cholera toxin B subunit (CTB) tracer that localizes to the plasma membrane only of labeled CSMNs. The main objective of this procedure is to provide CSMNs with a surface marker that can be used to immunopan only the labeled CSMNs by using a CTB-specific antibody.<\/jats:p>","DOI":"10.1101\/pdb.prot074922","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T13:38:19Z","timestamp":1396359499000},"page":"pdb.prot074922","source":"Crossref","is-referenced-by-count":3,"title":["Retrograde Labeling of Corticospinal Motor Neurons from Early Postnatal Rodents"],"prefix":"10.1101","volume":"2014","author":[{"given":"Wim","family":"Mandemakers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2014,4,1]]},"reference":[{"key":"2021111008472792000_2014.4.pdb.prot074922.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot074930"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot074922","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T19:07:42Z","timestamp":1636571262000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot074922"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":1,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2014,4,1]]},"published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1101\/pdb.prot074922"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot074922","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]}}}