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For retrograde infection of significant numbers of neurons, high virus titers are critical. Here we present reagents and a protocol for generating high-titer supernatants that can be concentrated 1000-fold for final titers in excess of 10<jats:sup>10<\/jats:sup> infectious units per milliliter. We demonstrate the usefulness of these vectors by selectively targeting corticothalamic and corticotectal neurons for high-level expression of a fluorophore in knock-in reporter mice.<\/jats:p>","DOI":"10.1101\/pdb.prot075879","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T17:06:12Z","timestamp":1427907972000},"page":"pdb.prot075879","source":"Crossref","is-referenced-by-count":6,"title":["Lentiviral Vectors for Retrograde Delivery of Recombinases and Transactivators"],"prefix":"10.1101","volume":"2015","author":[{"given":"Ian R.","family":"Wickersham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather A.","family":"Sullivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald M.","family":"Pao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Hamanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki A.","family":"Goosens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inder M.","family":"Verma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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