{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:01:05Z","timestamp":1790913665230,"version":"4.1.0"},"reference-count":4,"publisher":"Cold Spring Harbor Laboratory","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Chromosome conformation capture (3C) is a method for studying chromosomal organization that takes advantage of formaldehyde cross-linking to measure the spatial association of two pieces of chromatin. The 3C method begins with whole-cell formaldehyde fixation of chromatin. After cell lysis, solubilized chromatin is digested with a type II restriction endonuclease, and cross-linked DNA fragments are ligated together. Cross-links are reversed by degradation with proteinase K, and chimeric DNA molecules are purified by standard phenol:chloroform extraction. The resulting 3C library represents chromatin fragments that may be separated by large genomic distances or located on different chromosomes, but are close enough in three-dimensional space for cross-linking. Locus-specific oligonucleotide primers are used to detect interactions of interest in the 3C library using end-point polymerase chain reaction (PCR).<\/jats:p>","DOI":"10.1101\/pdb.prot085175","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T07:07:45Z","timestamp":1433228865000},"page":"pdb.prot085175","source":"Crossref","is-referenced-by-count":10,"title":["Chromosome Conformation Capture (3C) in Budding Yeast"],"prefix":"10.1101","volume":"2015","author":[{"given":"Jon-Matthew","family":"Belton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Job","family":"Dekker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2015,6,1]]},"reference":[{"key":"2021111009004260000_2015.6.pdb.prot085175.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot085191"},{"key":"2021111009004260000_2015.6.pdb.prot085175.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot085183"},{"key":"2021111009004260000_2015.6.pdb.prot085175.3","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth823"},{"key":"2021111009004260000_2015.6.pdb.prot085175.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.07.022"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot085175","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T19:27:08Z","timestamp":1636572428000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot085175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6]]},"references-count":4,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2015,6,1]]},"published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1101\/pdb.prot085175"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot085175","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6]]}}}