{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T13:26:54Z","timestamp":1748870814149},"reference-count":1,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>This protocol describes the recovery of replication-competent rabies viruses (RV) such as SAD L16 or SAD L16-eGFP. It is suggested that at least three parallel transfection experiments are performed to increase the success rate (in three 3.5-cm<jats:sup>2<\/jats:sup> dishes or in three wells in a six-well plate). The entire protocol takes 10 d, and successful rescue can be obtained after 6 d. An additional procedure for the recovery of G-gene-deleted viruses is also included. Please note that appropriate biosafety measures are needed.<\/jats:p>","DOI":"10.1101\/pdb.prot089409","type":"journal-article","created":{"date-parts":[[2015,12,2]],"date-time":"2015-12-02T16:12:54Z","timestamp":1449072774000},"page":"pdb.prot089409","source":"Crossref","is-referenced-by-count":3,"title":["Recovery of Replication-Competent and G-Gene-Deleted Rabies Viruses from cDNA"],"prefix":"10.1101","volume":"2015","author":[{"given":"Nadin","family":"Hagendorf","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl-Klaus","family":"Conzelmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2015,12,2]]},"reference":[{"key":"2021111009064630000_2015.12.pdb.prot089409.1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1128\/JVI.73.1.251-259.1999","article-title":"Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter","volume":"73","year":"1999","journal-title":"J Virol"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot089409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T19:30:26Z","timestamp":1636572626000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot089409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12]]},"references-count":1,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,12,2]]},"published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1101\/pdb.prot089409"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot089409","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12]]}}}