{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:45:22Z","timestamp":1790232322797,"version":"4.1.0"},"reference-count":1,"publisher":"Cold Spring Harbor Laboratory","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p>DNA is less soluble in solutions containing isopropanol than in solutions containing ethanol. In contrast to precipitation with ethanol, which requires 2\u20133 volumes of alcohol, precipitation with isopropanol is performed with 0.6\u20130.7 volume of alcohol. Isopropanol is often the better choice when precipitating DNA from large volumes of solution. Precipitation with isopropanol, described here, is performed at room temperature to lessen the risk that solutes like sucrose or sodium chloride will be coprecipitated with the DNA.<\/jats:p>","DOI":"10.1101\/pdb.prot093385","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T19:15:20Z","timestamp":1501614920000},"page":"pdb.prot093385","source":"Crossref","is-referenced-by-count":50,"title":["Precipitation of DNA with Isopropanol"],"prefix":"10.1101","volume":"2017","author":[{"given":"Michael R.","family":"Green","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Sambrook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2017,8,1]]},"reference":[{"key":"2021111009151533000_2017.8.pdb.prot093385.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot093377"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot093385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T19:50:18Z","timestamp":1636573818000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot093385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":1,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2017,8,1]]},"published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1101\/pdb.prot093385"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot093385","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}