{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:51:14Z","timestamp":1787010674082,"version":"build-2736575974"},"reference-count":11,"publisher":"Cold Spring Harbor Laboratory","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:p>\n                    This protocol describes the cross-linking of antibodies to either Protein A or G agarose beads using disuccinimidyl suberate (DSS), a bifunctional cross-linker capable of directly reacting with two different amines to form stable amide bonds. Proteins, including antibodies, generally display several primary amines in the side chains of lysine (K) residues and the amino terminus of each polypeptide that represent available potential targets for\n                    <jats:italic>N<\/jats:italic>\n                    -hydroxysuccinimide (NHS)\u2013ester cross-linking reagents. The antibody\u2013bead cross-linking process generates a reusable resource of antibody and beads, commonly referred to as an antibody-specific resin, and can be repeatedly used for the immunoprecipitation of specific proteins if treated and stored correctly.\n                  <\/jats:p>","DOI":"10.1101\/pdb.prot098632","type":"journal-article","created":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T14:51:04Z","timestamp":1549032664000},"page":"pdb.prot098632","source":"Crossref","is-referenced-by-count":6,"title":["Cross-Linking Antibodies to Beads with Disuccinimidyl Suberate (DSS)"],"prefix":"10.1101","volume":"2019","author":[{"given":"James","family":"DeCaprio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas O.","family":"Kohl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2019,2,1]]},"reference":[{"key":"2021111010123889000_2019.2.pdb.prot098632.1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.4161\/chan.4.4.12198","article-title":"Cross-linking of \u039bF508-CFTR promotes its trafficking to the plasma membrane","volume":"4","year":"2010","journal-title":"Channels"},{"key":"2021111010123889000_2019.2.pdb.prot098632.2","doi-asserted-by":"publisher","DOI":"10.1021\/ac901651r"},{"key":"2021111010123889000_2019.2.pdb.prot098632.3","doi-asserted-by":"publisher","DOI":"10.1021\/pr100898e"},{"key":"2021111010123889000_2019.2.pdb.prot098632.4","doi-asserted-by":"publisher","DOI":"10.1021\/ac902051q"},{"key":"2021111010123889000_2019.2.pdb.prot098632.5","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1360\/04yc0014","article-title":"Disuccinimidyl suberate cross-linked hemoglobin as a novel red blood cell substitute","volume":"48","year":"2005","journal-title":"Sci China C Life Sci"},{"key":"2021111010123889000_2019.2.pdb.prot098632.6","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1544"},{"key":"2021111010123889000_2019.2.pdb.prot098632.7","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1016\/j.jasms.2010.06.016","article-title":"Does chemical cross-linking with NHS esters reflect the chemical equilibrium of protein\u2013protein noncovalent interactions in solution?","volume":"21","year":"2010","journal-title":"J Am Soc Mass Spectrom"},{"key":"2021111010123889000_2019.2.pdb.prot098632.8","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/0022-1759(91)90014-7","article-title":"Optimization of binding capacity and specificity of protein G on various solid matrices for immunoglobulins","volume":"136","year":"1991","journal-title":"J Immunol Methods"},{"key":"2021111010123889000_2019.2.pdb.prot098632.9","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1280"},{"key":"2021111010123889000_2019.2.pdb.prot098632.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2011.05.010"},{"key":"2021111010123889000_2019.2.pdb.prot098632.11","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3109\/21691401.2012.762652","article-title":"Hemoglobin\u2013albumin cross-linking with disuccinimidyl suberate (DSS) and\/or glutaraldehyde for blood substitutes","volume":"42","year":"2014","journal-title":"Artif Cells Nanomed Biotechnol"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot098632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T15:09:07Z","timestamp":1636556947000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot098632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2,1]]},"published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1101\/pdb.prot098632"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot098632","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,2]]}}}