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The cross-linking process can be reversed, and protein-bound DNA fragments of optimal length ranging from 200 to 1000 base pairs (bp) can subsequently be purified and measured or sequenced by numerous analytical methods. In this protocol, two different fixation methods are described in detail. The first involves the standard fixation of cells and tissue by formaldehyde if the target antigen is highly abundant. The dual cross-linking procedure presented at the end includes an additional preformaldehyde cross-linking step and can be especially useful when the target protein is in low abundance or if it is indirectly associated with chromatin DNA through another protein.<\/jats:p>","DOI":"10.1101\/pdb.prot098665","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T22:28:09Z","timestamp":1596493689000},"page":"pdb.prot098665","source":"Crossref","is-referenced-by-count":14,"title":["Chromatin Immunoprecipitation"],"prefix":"10.1101","volume":"2020","author":[{"given":"James","family":"DeCaprio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas 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