{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T02:35:44Z","timestamp":1788489344270,"version":"build-2803163510"},"reference-count":17,"publisher":"Cold Spring Harbor Laboratory","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:p>Standardized rearing methods for the yellow fever mosquito<jats:italic>Aedes aegypti<\/jats:italic>are critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol for<jats:italic>Aedes aegypti<\/jats:italic>stocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feeding<jats:italic>Aedes aegypti<\/jats:italic>to yield viable eggs for continuous culture or storage, are detailed.<\/jats:p>","DOI":"10.1101\/pdb.prot108017","type":"journal-article","created":{"date-parts":[[2022,10,17]],"date-time":"2022-10-17T23:12:43Z","timestamp":1666048363000},"page":"pdb.prot108017","source":"Crossref","is-referenced-by-count":21,"title":["Batch Rearing<i>Aedes aegypti<\/i>"],"prefix":"10.1101","volume":"2023","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2672-0295","authenticated-orcid":false,"given":"Margot P.","family":"Wohl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8502-8712","authenticated-orcid":false,"given":"Conor J.","family":"McMeniman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"2023030505375858000_2023.3.pdb.prot108017.1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006142."},{"key":"2023030505375858000_2023.3.pdb.prot108017.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0221838"},{"key":"2023030505375858000_2023.3.pdb.prot108017.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3748"},{"key":"2023030505375858000_2023.3.pdb.prot108017.4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053816"},{"key":"2023030505375858000_2023.3.pdb.prot108017.5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087468"},{"key":"2023030505375858000_2023.3.pdb.prot108017.6","unstructured":"Gerberg EJ , Barnard DR , Ward RA . 1994. 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Care and maintenance of Aedes mosquito colonies. In The molecular biology of insect disease vectors: a methods manual (ed. Crampton JM , Beard CB , Louis C ), pp. 13\u201320. Springer Netherlands, Dordrecht.","DOI":"10.1007\/978-94-009-1535-0_2"},{"key":"2023030505375858000_2023.3.pdb.prot108017.15","unstructured":"Munstermann LE , Wasmuth LM . 1985. Aedes aegypti . In Handbook of insect rearing (ed. Singh P , Moore RF ), pp. 7\u201314. 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