{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T05:25:51Z","timestamp":1735881951172,"version":"3.32.0"},"reference-count":2,"publisher":"Cold Spring Harbor Laboratory","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>Chromatin immunoprecipitation (ChIP) is a well-characterized procedure used to reveal specific patterns of protein\u2013DNA interactions and identify the binding sites of proteins on DNA. ChIP has been used to study many aspects of<jats:italic>Drosophila<\/jats:italic>biology, including neurobiology. This protocol describes in detail how to prepare cross-linked chromatin from<jats:italic>Drosophila<\/jats:italic>antennae and brains followed by immunoprecipitation (X-ChIP). We first describe tissue dissection, chromatin cross-linking with formaldehyde, quenching of the cross-linking, homogenization of tissues, and sonication for shearing the chromatin. Additionally, we describe how to optimize the sonication efficiency and fixation time and concentration using<jats:italic>Drosophila<\/jats:italic>brain samples as an example. These parameters are crucial for successful ChIP.<\/jats:p>","DOI":"10.1101\/pdb.prot108140","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T17:09:51Z","timestamp":1716484191000},"page":"pdb.prot108140","source":"Crossref","is-referenced-by-count":0,"title":["Sample Preparation for Chromatin Immunoprecipitation (ChIP) from<i>Drosophila<\/i>Antennal and Brain Samples"],"prefix":"10.1101","volume":"2025","author":[{"given":"Chengcheng","family":"Du","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pelin","family":"Volkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2024,3,7]]},"reference":[{"key":"2025010208250984000_2025.1.pdb.prot108140.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot108142"},{"key":"2025010208250984000_2025.1.pdb.prot108140.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot066878"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot108140","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,2]],"date-time":"2025-01-02T16:25:45Z","timestamp":1735835145000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot108140"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,7]]},"references-count":2,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1,2]]},"published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1101\/pdb.prot108140"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot108140","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"type":"print","value":"1940-3402"},{"type":"electronic","value":"1559-6095"}],"subject":[],"published":{"date-parts":[[2024,3,7]]}}}