{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:38:54Z","timestamp":1740145134970,"version":"3.37.3"},"reference-count":5,"publisher":"Cold Spring Harbor Laboratory","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p><jats:italic>Anopheles gambiae<\/jats:italic>is a highly anthropophilic (human-preferring) malaria vector that prefers to blood feed frequently and selectively on humans. This mosquito species exhibits a strong innate olfactory preference to seek out human scent over other animals, and certain humans over others\u2014key behavioral traits with the potential to drive heterogeneity in biting risk and malaria transmission. Here, we describe the application of a large-scale, semi-field system in Zambia for the quantification of<jats:italic>An. gambiae<\/jats:italic>olfactory preferences toward whole body odor sourced from individual humans. We detail steps for modifying one-person canvas tents to duct odor from sleeping humans into a central, semi-field flight cage arena that is securely screened. Using this system, we describe a protocol to perform two-choice olfactory preference assays with two human volunteers using laboratory-reared<jats:italic>An. gambiae<\/jats:italic>and odor-guided thermotaxis assays that leverage infrared video tracking to quantify mosquito landings on heated targets baited with each body odor sample. This multichoice olfactory preference assay has the potential to be applied with expanded cohorts of humans to define the chemosensory basis of<jats:italic>An. gambiae<\/jats:italic>host preference and interindividual differences in human attractiveness to mosquitoes and to be used to quantify the effects of protective measures such as personal and spatial repellents on mosquito landing behavior.<\/jats:p>","DOI":"10.1101\/pdb.prot108304","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T16:35:12Z","timestamp":1711989312000},"page":"pdb.prot108304","source":"Crossref","is-referenced-by-count":3,"title":["Quantification of<i>Anopheles gambiae<\/i>Olfactory Preferences under Semi-Field Conditions"],"prefix":"10.1101","volume":"2024","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6552-3297","authenticated-orcid":false,"given":"Diego","family":"Giraldo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8502-8712","authenticated-orcid":false,"given":"Conor J.","family":"McMeniman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"2024040109350596000_2024.4.pdb.prot108304.1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-7-158"},{"key":"2024040109350596000_2024.4.pdb.prot108304.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot108303"},{"key":"2024040109350596000_2024.4.pdb.prot108304.3","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-1-19"},{"key":"2024040109350596000_2024.4.pdb.prot108304.4","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3688-0"},{"key":"2024040109350596000_2024.4.pdb.prot108304.5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000988"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot108304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T16:35:41Z","timestamp":1711989341000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot108304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,23]]},"references-count":5,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,4,1]]},"published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1101\/pdb.prot108304"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot108304","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"type":"print","value":"1940-3402"},{"type":"electronic","value":"1559-6095"}],"subject":[],"published":{"date-parts":[[2023,8,23]]}}}