{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:19:24Z","timestamp":1717460364701},"reference-count":6,"publisher":"Cold Spring Harbor Laboratory","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:p>The selection of mosquito transgenic larvae using a nonfluorescent approach can be advantageous to reserve fluorophores for downstream applications, such as immunostaining or for the study of promoter activity by cloning a fluorescence reporter gene under the control of that promoter. We previously reported that puromycin selection is efficient in transgenic<jats:italic>Anopheles gambiae<\/jats:italic>or<jats:italic>Anopheles coluzzii<\/jats:italic>larvae expressing an<jats:italic>OpIE2-pac<\/jats:italic>selection marker. A concentration of puromycin of &gt;10 \u00b5g\/mL is lethal for<jats:italic>Anopheles<\/jats:italic>larvae, unless they carry the resistance gene, conferring them resistance to puromycin concentrations of 25\u201380 \u00b5g\/mL. A drawback of this fully dominant selection marker is that, unlike with fluorescence markers, homozygous transgenics cannot be distinguished from heterozygotes. Here, we outline the procedure for selecting puromycin-resistant transgenic<jats:italic>Anopheles<\/jats:italic>larvae.<\/jats:p>","DOI":"10.1101\/pdb.prot108308","type":"journal-article","created":{"date-parts":[[2024,5,23]],"date-time":"2024-05-23T17:15:57Z","timestamp":1716484557000},"page":"pdb.prot108308","source":"Crossref","is-referenced-by-count":1,"title":["Selecting Transgenic Mosquito Larvae with Puromycin"],"prefix":"10.1101","volume":"2024","author":[{"given":"Eric","family":"Marois","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2023,9,11]]},"reference":[{"key":"2024060308300733000_2024.6.pdb.prot108308.1","unstructured":"Benedict MQ . 2015. Methods in Anopheles Research\u2014MR4; https:\/\/www.beiresources.org\/portals\/2\/MR4\/MR4_Publications\/Methods%20in%20Anopheles%20Research%202014\/2014MethodsinAnophelesResearchManualFullVersionv2tso.pdf [Accessed August 4, 2023]"},{"key":"2024060308300733000_2024.6.pdb.prot108308.2","doi-asserted-by":"publisher","DOI":"10.3791\/62591"},{"key":"2024060308300733000_2024.6.pdb.prot108308.3","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-026-7_41"},{"key":"2024060308300733000_2024.6.pdb.prot108308.4","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.top107694"},{"key":"2024060308300733000_2024.6.pdb.prot108308.5","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2014.117"},{"key":"2024060308300733000_2024.6.pdb.prot108308.6","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.016808"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot108308","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T15:31:10Z","timestamp":1717428670000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot108308"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,11]]},"references-count":6,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6,3]]},"published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1101\/pdb.prot108308"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot108308","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,11]]}}}