{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T06:07:23Z","timestamp":1792822043827,"version":"4.5.0"},"reference-count":4,"publisher":"Cold Spring Harbor Laboratory","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>INTRODUCTION<\/jats:title>\n                  <jats:p>Bone marrow-derived macrophages (BMM) are primary macrophage cells, derived from bone marrow cells in vitro in the presence of growth factors. Macrophage colony-stimulating factor (M-CSF) is a lineage-specific growth factor that is responsible for the proliferation and differentiation of committed myeloid progenitors into cells of the macrophage\/monocyte lineage. Mice lacking functional M-CSF are deficient in macrophages and osteoclasts and suffer from osteopetrosis. In this protocol, bone marrow cells are grown in culture dishes in the presence of M-CSF, which is secreted by L929 cells and is used in the form of L929-conditioned medium. Under these conditions, the bone marrow monocyte\/macrophage progenitors will proliferate and differentiate into a homogenous population of mature BMMs. The efficiency of the differentiation is assessed using fluorescence-activated cell sorting (FACS) analysis of Mac-1 and 4\/80 surface antigen expression. Once differentiated, the BMMs are suitable for numerous types of experimental manipulations, including morphological, gene expression, and physiological studies. For example, phagocytic cells such as macrophages have a unique ability to ingest microbes. We describe a test for the phagocytic efficiency of BMMs by exposing them to fluorescently labeled yeast zymosan bioparticles. Also, a method to deliver DNA or small interfering RNAs (siRNAs) into these hard-to-transfect cells is described. Finally, the proliferation of the BMMs is assayed using carboxyfluorescein succinimidyl ester (CFSE), a fluorescein derivative that partitions equally between daughter cells after cell division.<\/jats:p>","DOI":"10.1101\/pdb.prot5080","type":"journal-article","created":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T10:18:14Z","timestamp":1228126694000},"page":"pdb.prot5080","source":"Crossref","is-referenced-by-count":483,"title":["Bone Marrow-Derived Macrophages (BMM): Isolation and Applications"],"prefix":"10.1101","volume":"2008","author":[{"given":"Joachim","family":"Weischenfeldt","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Porse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2010,12,3]]},"reference":[{"key":"2021111008054723000_2008.12.pdb.prot5080.1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00756-06"},{"key":"2021111008054723000_2008.12.pdb.prot5080.2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031237"},{"key":"2021111008054723000_2008.12.pdb.prot5080.3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318580"},{"key":"2021111008054723000_2008.12.pdb.prot5080.4","doi-asserted-by":"publisher","DOI":"10.1101\/gad.468808"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot5080","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T12:17:23Z","timestamp":1636546643000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot5080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":4,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2010,12,3]]},"published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1101\/pdb.prot5080"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot5080","relation":{},"ISSN":["1940-3402","1559-6095","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"},{"value":"1559-6095","type":"print"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}