{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T19:51:35Z","timestamp":1760385095670},"reference-count":8,"publisher":"Cold Spring Harbor Laboratory","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>INTRODUCTION<\/jats:title><jats:p>Blood-feeding mosquitoes, including the dengue and yellow fever vector <jats:italic>Aedes aegypti<\/jats:italic>, transmit many of the world\u2019s deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol describes a method for fixation and dissection of <jats:italic>Ae. aegypti<\/jats:italic> embryos, larvae, and pupae. Tissue processed in this manner can be used subsequently for in situ hybridization detection of mRNA or immunohistochemical analysis of protein expression.<\/jats:p>","DOI":"10.1101\/pdb.prot5508","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T14:23:09Z","timestamp":1285942989000},"page":"pdb.prot5508","source":"Crossref","is-referenced-by-count":15,"title":["Fixation and Preparation of Developing Tissues from <i>Aedes aegypti<\/i>: Figure 1."],"prefix":"10.1101","volume":"2010","author":[{"given":"Anthony","family":"Clemons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morgan","family":"Haugen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Flannery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristopher","family":"Kast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin","family":"Jacowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Severson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly","family":"Duman-Scheel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2010,10,1]]},"reference":[{"key":"2021111008193355000_2010.10.pdb.prot5508.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5507"},{"key":"2021111008193355000_2010.10.pdb.prot5508.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5510"},{"key":"2021111008193355000_2010.10.pdb.prot5508.3","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.emo141"},{"key":"2021111008193355000_2010.10.pdb.prot5508.4","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1525-142X.2007.00144.x","article-title":"Delayed onset of midline netrin expression in Artemia franciscana coincides with commissural axon growth and provides evidence for homology of midline cells in distantly related arthropods","volume":"9","author":"Duman-Scheel","year":"2007","journal-title":"Evol Dev"},{"key":"2021111008193355000_2010.10.pdb.prot5508.5","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5509"},{"key":"2021111008193355000_2010.10.pdb.prot5508.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(08)60927-9"},{"key":"2021111008193355000_2010.10.pdb.prot5508.7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1242\/dev.107.2.201","article-title":"Expression of engrailed during segmentation in grasshopper and crayfish","volume":"107","author":"Patel","year":"1989","journal-title":"Development"},{"key":"2021111008193355000_2010.10.pdb.prot5508.8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-142X.2009.00328.x"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot5508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T18:16:01Z","timestamp":1636568161000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot5508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":8,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2010,10,1]]},"published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1101\/pdb.prot5508"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot5508","relation":{},"ISSN":["1940-3402","1559-6095","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"},{"value":"1559-6095","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10]]}}}