{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:44:40Z","timestamp":1760820280471},"reference-count":8,"publisher":"Cold Spring Harbor Laboratory","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>INTRODUCTION<\/jats:title><jats:p>Blood-feeding mosquitoes, including the dengue and yellow fever vector <jats:italic>Aedes aegypti<\/jats:italic>, transmit many of the world\u2019s deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol for whole-mount in situ hybridization can be used to analyze gene expression in <jats:italic>Ae. aegypti<\/jats:italic> embryos and larvae, a critical aspect of understanding developmental gene function in this vector mosquito.<\/jats:p>","DOI":"10.1101\/pdb.prot5509","type":"journal-article","created":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T14:23:09Z","timestamp":1285942989000},"page":"pdb.prot5509","source":"Crossref","is-referenced-by-count":29,"title":["Whole-Mount In Situ Hybridization for Analysis of Gene Expression during <i>Aedes aegypti<\/i> Development: Figure 1."],"prefix":"10.1101","volume":"2010","author":[{"given":"Morgan","family":"Haugen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Tomchaney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristopher","family":"Kast","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen","family":"Flannery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Clemons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin","family":"Jacowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Simanton Holland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christy","family":"Le","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Severson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Molly","family":"Duman-Scheel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2010,10,1]]},"reference":[{"key":"2021111008193356000_2010.10.pdb.prot5509.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5508"},{"key":"2021111008193356000_2010.10.pdb.prot5509.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5510"},{"key":"2021111008193356000_2010.10.pdb.prot5509.3","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.emo141"},{"key":"2021111008193356000_2010.10.pdb.prot5509.4","doi-asserted-by":"publisher","DOI":"10.1007\/s00427-002-0215-6"},{"key":"2021111008193356000_2010.10.pdb.prot5509.5","unstructured":"Patel N (1996) in A laboratory guide to RNA: Isolation, analysis, and synthesis, In situ hybridization to whole mount Drosophila embryos, ed Krieg PA (Wiley-Liss, New York), pp 357\u2013370."},{"key":"2021111008193356000_2010.10.pdb.prot5509.6","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.1242\/dev.128.18.3459","article-title":"Grasshopper hunchback expression reveals conserved and novel aspects of axis formation and segmentation","volume":"128","author":"Patel","year":"2001","journal-title":"Development"},{"key":"2021111008193356000_2010.10.pdb.prot5509.7","doi-asserted-by":"publisher","DOI":"10.1007\/BF00291041"},{"key":"2021111008193356000_2010.10.pdb.prot5509.8","doi-asserted-by":"crossref","first-page":"323","DOI":"10.4161\/fly.7230","article-title":"Whole mount in situ hybridization detection of mRNA in GFP-marked Drosophila imaginal disc mosaic clones","volume":"2","author":"Van Zomeren-Dohm","year":"2008","journal-title":"Fly"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.prot5509","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T18:15:56Z","timestamp":1636568156000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.prot5509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":8,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2010,10,1]]},"published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1101\/pdb.prot5509"],"URL":"https:\/\/doi.org\/10.1101\/pdb.prot5509","relation":{},"ISSN":["1940-3402","1559-6095","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"},{"value":"1559-6095","type":"print"}],"subject":[],"published":{"date-parts":[[2010,10]]}}}