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We have developed an immunopanning protocol to specifically purify pericytes from capillaries in the rodent optic nerve. This method relies on a combination of negative and positive selection criteria and allows prospective, acute isolation of pericytes. Use of this method will facilitate studies of pericyte cell biology and function and pericyte\u2013endothelial cell interactions.<\/jats:p>","DOI":"10.1101\/pdb.top070888","type":"journal-article","created":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T13:45:27Z","timestamp":1401716727000},"page":"pdb.top070888","source":"Crossref","is-referenced-by-count":3,"title":["Purification and Culture of Central Nervous System Pericytes"],"prefix":"10.1101","volume":"2014","author":[{"given":"Lu","family":"Zhou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabien","family":"Sohet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Daneman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"246","published-online":{"date-parts":[[2014,6,2]]},"reference":[{"key":"2021111008545495000_2014.6.pdb.top070888.1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09522"},{"key":"2021111008545495000_2014.6.pdb.top070888.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.07.001"},{"key":"2021111008545495000_2014.6.pdb.top070888.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.09.043"},{"key":"2021111008545495000_2014.6.pdb.top070888.4","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.82.2.221"},{"key":"2021111008545495000_2014.6.pdb.top070888.5","doi-asserted-by":"publisher","DOI":"10.1186\/2045-8118-8-8"},{"key":"2021111008545495000_2014.6.pdb.top070888.6","doi-asserted-by":"publisher","DOI":"10.1038\/nature09513"},{"key":"2021111008545495000_2014.6.pdb.top070888.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2005.01.027"},{"key":"2021111008545495000_2014.6.pdb.top070888.8","first-page":"375","article-title":"Isolation and characterization of cerebral microvascular pericytes","volume":"89","year":"2003","journal-title":"Methods Mol Med"},{"key":"2021111008545495000_2014.6.pdb.top070888.9","doi-asserted-by":"publisher","DOI":"10.2174\/138161208784705469"},{"key":"2021111008545495000_2014.6.pdb.top070888.10","doi-asserted-by":"publisher","DOI":"10.1006\/mvre.2000.2244"},{"key":"2021111008545495000_2014.6.pdb.top070888.11","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600272"},{"key":"2021111008545495000_2014.6.pdb.top070888.12","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1242\/dev.128.7.1059","article-title":"The cephalic neural crest provides pericytes and smooth muscle cells to all blood vessels of the face and forebrain","volume":"128","year":"2001","journal-title":"Development"},{"key":"2021111008545495000_2014.6.pdb.top070888.13","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.161521"},{"key":"2021111008545495000_2014.6.pdb.top070888.14","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-003-0745-x"},{"key":"2021111008545495000_2014.6.pdb.top070888.15","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0177(200007)218:3<472::AID-DVDY1008>3.0.CO;2-#"},{"key":"2021111008545495000_2014.6.pdb.top070888.16","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203165"},{"key":"2021111008545495000_2014.6.pdb.top070888.17","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.10.3107"},{"key":"2021111008545495000_2014.6.pdb.top070888.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.regpep.2004.05.023"},{"key":"2021111008545495000_2014.6.pdb.top070888.19","doi-asserted-by":"crossref","first-page":"3047","DOI":"10.1242\/dev.126.14.3047","article-title":"Role of PDGF-B and PDGFR-\u03b2 in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse","volume":"126","year":"1999","journal-title":"Development"},{"key":"2021111008545495000_2014.6.pdb.top070888.20","doi-asserted-by":"crossref","unstructured":"Korn J , Christ B , Kurz H . 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