{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:50:03Z","timestamp":1790106603725,"version":"4.0.1"},"reference-count":7,"publisher":"Cold Spring Harbor Laboratory","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cold Spring Harb Protoc"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:p>Collecting eggs from adult<jats:italic>Xenopus laevis<\/jats:italic>and<jats:italic>Xenopus tropicalis<\/jats:italic>to raise healthy embryos and tadpoles is relatively simple but requires careful handling of the frog. Eggs can be fertilized through natural matings or by in vitro fertilization and examined visually. Here we review how eggs are obtained and how to recognize healthy eggs that will develop into high-quality embryos.<\/jats:p>","DOI":"10.1101\/pdb.top106195","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T16:35:40Z","timestamp":1683045340000},"page":"pdb.top106195","source":"Crossref","is-referenced-by-count":5,"title":["Obtaining<i>Xenopus<\/i>Eggs and Embryos"],"prefix":"10.1101","volume":"2023","author":[{"given":"Hazel L.","family":"Sive","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8247-4880","authenticated-orcid":false,"given":"Richard M.","family":"Harland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"246","published-online":{"date-parts":[[2022,10,25]]},"reference":[{"key":"2023051910450844000_2023.5.pdb.top106195.1","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106609"},{"key":"2023051910450844000_2023.5.pdb.top106195.2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106351"},{"key":"2023051910450844000_2023.5.pdb.top106195.3","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106344"},{"key":"2023051910450844000_2023.5.pdb.top106195.4","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106252"},{"key":"2023051910450844000_2023.5.pdb.top106195.5","unstructured":"Nieuwkoop PD , Faber J . 1967. Normal table of Xenopus laevis (Daudin): a systematical and chronological survey of the development from the fertilized egg till the end of morphogenesis. North-Holland Publishing Company, Amsterdam, reprinted 1994 Garland Publishing, New York. ebook 2020 at https:\/\/doi.org\/10.1201\/9781003064565 with figures at https:\/\/www.xenbase.org\/anatomy\/alldev.do."},{"key":"2023051910450844000_2023.5.pdb.top106195.6","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106203"},{"key":"2023051910450844000_2023.5.pdb.top106195.7","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot106211"}],"container-title":["Cold Spring Harbor Protocols"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1101\/pdb.top106195","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T17:45:34Z","timestamp":1684518334000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.cshprotocols.org\/lookup\/doi\/10.1101\/pdb.top106195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,25]]},"references-count":7,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2023,5,2]]},"published-print":{"date-parts":[[2023,5]]}},"alternative-id":["10.1101\/pdb.top106195"],"URL":"https:\/\/doi.org\/10.1101\/pdb.top106195","relation":{},"ISSN":["1940-3402","1559-6095"],"issn-type":[{"value":"1940-3402","type":"print"},{"value":"1559-6095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,25]]}}}