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Cytogenetic approaches assist with the development of chromosome-scale genome assemblies that are useful tools for studying mosquito biology and for designing novel vector control strategies. Fluorescence in situ hybridization is a powerful technique for localizing specific DNA sequences within the linear chromosome structure and within the spatial organization of the cell nucleus. Here, we introduce protocols used in our laboratories for chromosome visualization and their application in mosquitoes.<\/jats:p>","DOI":"10.1101\/pdb.top107732","type":"journal-article","created":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T06:28:01Z","timestamp":1672986481000},"page":"pdb.top107732","source":"Crossref","is-referenced-by-count":1,"title":["Visualization of the Linear and Spatial Organization of Chromosomes in Mosquitoes"],"prefix":"10.1101","volume":"2022","author":[{"given":"Jiangtao","family":"Liang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon M.","family":"Bondarenko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0752-3747","authenticated-orcid":false,"given":"Igor 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