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We found that blockage of the alpha4\/beta1 integrin (Itga4\/Itgb1) selectively hampered binding of mIgG3 to macrophages and mIgG3-mediated phagocytosis. Manganese, an integrin activator, increased mIgG3 binding to macrophages. Blockage of Fc\u03b3RI or Itgb1 inhibited binding of different mIgG3 antibodies to variable extents. Our results indicate an integrin component in the mIgG3 receptor. Given the more ancient origin of integrins in comparison with Fc\u03b3R, this observation could have far ranging implications for our understanding of the evolution of antibody-mediated immunity, as well as in immunity to microorganisms, pathogenesis of autoimmune diseases and antibody engineering.<\/jats:p>","DOI":"10.1101\/207274","type":"posted-content","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T11:44:06Z","timestamp":1508759046000},"source":"Crossref","is-referenced-by-count":0,"title":["Integrin alpha 4 \/ beta 1 (CD49d\/CD29) is a component of the murine IgG3 receptor"],"prefix":"10.64898","author":[{"given":"Carolyn","family":"Saylor Hawk","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7523-3031","authenticated-orcid":false,"given":"Carolina","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Diane Sthefany","family":"Lima de Oliveira","sequence":"additional","affiliation":[]},{"given":"Verenice","family":"Paredes","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Albuquerque","sequence":"additional","affiliation":[]},{"given":"Anam\u00e9lia","family":"Lorenzetti Bocca","sequence":"additional","affiliation":[]},{"given":"Anan\u00e9sia","family":"Correa dos Santos","sequence":"additional","affiliation":[]},{"given":"Victoria","family":"Rusakova","sequence":"additional","affiliation":[]},{"given":"Heather","family":"Holemon","sequence":"additional","affiliation":[]},{"given":"Maria Sueli","family":"Soares Felipe","sequence":"additional","affiliation":[]},{"given":"Hideo","family":"Yagita","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8656-5835","authenticated-orcid":false,"given":"Andr\u00e9 Moraes","family":"Nicola","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-9167","authenticated-orcid":false,"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019071715041247000_207274v1.1","doi-asserted-by":"crossref","first-page":"526","DOI":"10.4049\/jimmunol.143.2.526","article-title":"IgG3 is the major source of cryoglobulins in mice","volume":"143","year":"1989","journal-title":"J Immunol"},{"key":"2019071715041247000_207274v1.2","doi-asserted-by":"crossref","unstructured":"Anthony, R.M. , Kobayashi, T. , Wermeling, F. , and Ravetch, J.V. 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