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Coupling comparative genomics with biochemical and genetic approaches, we show that the alcoholic fermentation pathway was profoundly remodeled in the W\/S clade, as genes required for alcoholic fermentation were lost and subsequently re-acquired from bacteria through horizontal gene transfer. We further show that the reinstated fermentative pathway is functional and that an enzyme required for sucrose assimilation is also of bacterial origin, reinforcing the adaptive nature of the genetic novelties identified in the W\/S clade. This work shows how even central carbon metabolism can be remodeled by a surge of HGT events.\n                <\/jats:p>","DOI":"10.1101\/213686","type":"posted-content","created":{"date-parts":[[2017,11,4]],"date-time":"2017-11-04T01:10:12Z","timestamp":1509757812000},"source":"Crossref","is-referenced-by-count":0,"title":["Evidence for loss and adaptive reacquisition of alcoholic fermentation in an early-derived fructophilic yeast lineage"],"prefix":"10.64898","author":[{"given":"Carla","family":"Gon\u00e7alves","sequence":"first","affiliation":[]},{"given":"Jennifer H.","family":"Wisecaver","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5668-6705","authenticated-orcid":false,"given":"Madalena","family":"Salema-Oom","sequence":"additional","affiliation":[]},{"given":"Maria Jos\u00e9","family":"Leandro","sequence":"additional","affiliation":[]},{"given":"Xing-Xing","family":"Shen","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9912-8802","authenticated-orcid":false,"given":"David","family":"Peris","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5088-7461","authenticated-orcid":false,"given":"Chris Todd","family":"Hittinger","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7248-6551","authenticated-orcid":false,"given":"Antonis","family":"Rokas","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2103-1060","authenticated-orcid":false,"given":"Paula","family":"Gon\u00e7alves","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019071802234247000_213686v1.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1517242113"},{"key":"2019071802234247000_213686v1.2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.000154"},{"key":"2019071802234247000_213686v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evs011"},{"key":"2019071802234247000_213686v1.4","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3517"},{"key":"2019071802234247000_213686v1.5","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"2019071802234247000_213686v1.6","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.5.11.2894"},{"issue":"1","key":"2019071802234247000_213686v1.7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/genetics\/98.1.25","article-title":"Mutants of yeast defective in sucrose utilization","volume":"98","year":"1981","journal-title":"Genetics"},{"key":"2019071802234247000_213686v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3876"},{"issue":"4","key":"2019071802234247000_213686v1.9","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1128\/AEM.64.4.1350-1358.1998","article-title":"Pichia stipitis Genes for Alcohol Dehydrogenase with Fermentative and Respiratory Functions","volume":"64","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"2019071802234247000_213686v1.10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003587"},{"key":"2019071802234247000_213686v1.11","doi-asserted-by":"publisher","DOI":"10.1111\/1567-1364.12161"},{"key":"2019071802234247000_213686v1.12","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2008.00387.x"},{"key":"2019071802234247000_213686v1.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2011.00760.x"},{"key":"2019071802234247000_213686v1.14","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/fox054"},{"key":"2019071802234247000_213686v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2014.02.004"},{"key":"2019071802234247000_213686v1.16","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.7.3909-3917.1990"},{"key":"2019071802234247000_213686v1.17","doi-asserted-by":"publisher","DOI":"10.1142\/9781848165632_0019"},{"key":"2019071802234247000_213686v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2009.08.002"},{"key":"2019071802234247000_213686v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2013.06.002"},{"key":"2019071802234247000_213686v1.20","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-013-0506-3"},{"key":"2019071802234247000_213686v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2015.01.009"},{"issue":"9","key":"2019071802234247000_213686v1.22","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1128\/AEM.63.9.3399-3404.1997","article-title":"Metabolic responses of pyruvate decarboxylase-negative Saccharomyces cerevisiae to glucose excess","volume":"63","year":"1997","journal-title":"Appl Environ Microbiol"},{"issue":"8","key":"2019071802234247000_213686v1.23","doi-asserted-by":"crossref","first-page":"3754","DOI":"10.1016\/S0021-9258(18)61419-X","article-title":"Kinetic characterization of yeast alcohol dehydrogenases. 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