{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T12:58:57Z","timestamp":1768481937503,"version":"3.49.0"},"posted":{"date-parts":[[2018,5,1]]},"group-title":"Cell Biology","reference-count":35,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2018,5,1]]},"abstract":"<jats:title>Summary statement<\/jats:title>\n                <jats:p>\n                  PLK4 binds to microtubules and self assembles into supramolecular assemblies that recruit tubulin and trigger\n                  <jats:italic>de novo<\/jats:italic>\n                  MTOC formation in\n                  <jats:italic>Xenopus laevis<\/jats:italic>\n                  extracts.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The centrosome is an important microtubule-organizing center (MTOCs) in animal cells and it consists of two barrel-shaped structures (centrioles), surrounded by the pericentriolar material (PCM), which nucleates microtubules. PCM components form condensates, supramolecular assemblies that concentrate microtubule nucleators. Centrosomes can form close to an existing structure (canonical duplication) or\n                    <jats:italic>de novo<\/jats:italic>\n                    . How centrosomes form de novo is not known. PLK4 is a master driver of centrosome biogenesis, which is critical to recruit several centriole components. Here, we investigate the beginning of centrosome biogenesis, taking advantage of\n                    <jats:italic>Xenopus egg<\/jats:italic>\n                    extracts, where we and others have shown that PLK4 can induce\n                    <jats:italic>de novo<\/jats:italic>\n                    MTOC formation (Eckerdt et al., 2011; Zitouni et al., 2016). Surprisingly, we observe that\n                    <jats:italic>in vitro,<\/jats:italic>\n                    PLK4 can self-assemble into supramolecular assemblies that recruit \u03b1\/\u03b2-tubulin. In\n                    <jats:italic>Xenopus<\/jats:italic>\n                    extracts, PLK4 supramolecular assemblies additionally recruit the PLK4 substrate STIL and the microtubule nucleator, \u03b3-tubulin, and form acentriolar MTOCs\n                    <jats:italic>de novo<\/jats:italic>\n                    . The assembly of these robust microtubule asters is independent of dynein, similarly to centrosomes. We suggest a new mechanism of action for PLK4, where it forms a self-organizing catalytic scaffold that recruits centriole components, PCM factors and \u03b1\/\u03b2-tubulin, leading to MTOC formation.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.1101\/312272","type":"posted-content","created":{"date-parts":[[2018,5,2]],"date-time":"2018-05-02T01:45:12Z","timestamp":1525225512000},"source":"Crossref","is-referenced-by-count":3,"title":["PLK4 is a microtubule-associated protein that self assembles promoting\n                  <i>de novo<\/i>\n                  MTOC formation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1220-410X","authenticated-orcid":false,"given":"Susana Montenegro","family":"Gouveia","sequence":"first","affiliation":[]},{"given":"Sihem","family":"Zitouni","sequence":"additional","affiliation":[]},{"given":"Dong","family":"Kong","sequence":"additional","affiliation":[]},{"given":"Paulo","family":"Duarte","sequence":"additional","affiliation":[]},{"given":"Beatriz Ferreira","family":"Gomes","sequence":"additional","affiliation":[]},{"given":"Ana Laura","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Erin M.","family":"Tranfield","sequence":"additional","affiliation":[]},{"given":"Antony","family":"Hyman","sequence":"additional","affiliation":[]},{"given":"Jadranka","family":"Loncarek","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1987-5598","authenticated-orcid":false,"given":"Monica","family":"Bettencourt-Dias","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019071909422300000_312272v1.1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093930"},{"key":"2019071909422300000_312272v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.11.042"},{"key":"2019071909422300000_312272v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/35030148"},{"key":"2019071909422300000_312272v1.4","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970280206"},{"key":"2019071909422300000_312272v1.5","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201606077"},{"key":"2019071909422300000_312272v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.09.029"},{"issue":"Pt 11","key":"2019071909422300000_312272v1.7","first-page":"1477","article-title":"Focusing on spindle poles","volume":"111","year":"1998","journal-title":"J Cell Sci"},{"key":"2019071909422300000_312272v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.01.072"},{"key":"2019071909422300000_312272v1.9","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.138.5.1055"},{"key":"2019071909422300000_312272v1.10","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.2.399"},{"key":"2019071909422300000_312272v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1335"},{"key":"2019071909422300000_312272v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.04.065"},{"key":"2019071909422300000_312272v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2013.12.001"},{"key":"2019071909422300000_312272v1.14","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2015.03.013"},{"key":"2019071909422300000_312272v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2591"},{"key":"2019071909422300000_312272v1.16","unstructured":"Loncarek, J. and Bettencourt-Dias, M. 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