{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:53:46Z","timestamp":1768478026659,"version":"3.49.0"},"posted":{"date-parts":[[2018,5,18]]},"group-title":"Microbiology","reference-count":19,"publisher":"openRxiv","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2018,5,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Leishmaniasis is an important vector-borne neglected tropical disease caused by\n                  <jats:italic>Leishmania<\/jats:italic>\n                  parasites. Current anti-\n                  <jats:italic>Leishmania<\/jats:italic>\n                  chemotherapy is unsatisfactory, justifying the continued search for alternative treatment options. Herein, we propose the use of a minimally invasive bioluminescence-based murine model for preliminary\n                  <jats:italic>in vivo<\/jats:italic>\n                  screening of compounds against visceral infection by\n                  <jats:italic>Leishmania infantum<\/jats:italic>\n                  . We demonstrate that luciferase-expressing axenic amastigotes, unlike promastigotes, are highly infectious to BALB\/c mice and generate a robust bioluminescent signal in the main target organs, such as the liver and spleen. Finally, we validate the use of this technique to evaluate\n                  <jats:italic>in vivo<\/jats:italic>\n                  treatment efficacy using reference drugs amphotericin B and miltefosine.\n                <\/jats:p>","DOI":"10.1101\/326355","type":"posted-content","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T18:25:11Z","timestamp":1526667911000},"source":"Crossref","is-referenced-by-count":2,"title":["Whole-mouse\n                  <i>in vivo<\/i>\n                  bioluminescence imaging applied to drug screening against\n                  <i>Leishmania infantum<\/i>\n                  : a reliable method to evaluate efficacy and optimize treatment regimens"],"prefix":"10.64898","author":[{"given":"David M.","family":"Costa","sequence":"first","affiliation":[]},{"given":"Pedro","family":"Cec\u00edlio","sequence":"additional","affiliation":[]},{"given":"Nuno","family":"Santar\u00e9m","sequence":"additional","affiliation":[]},{"given":"Anabela","family":"Cordeiro-da-Silva","sequence":"additional","affiliation":[]},{"given":"Joana","family":"Tavares","sequence":"additional","affiliation":[]}],"member":"54368","reference":[{"key":"2019071911071035000_326355v1.1","unstructured":"WHO. 2017 Leishmaniasis, Fact sheet No 375 http:\/\/www.who.int\/mediacentre\/factsheets\/fs375\/en\/. 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